以氧化乐果为模板分子,甲基丙烯酸（MAA）为功能单体,利用悬浮聚合法制备了粒径分布范围较窄的氧化乐果分子印迹微球（MIPMs）和空白对照微球（NMIPMs）。通过紫外光谱及红外光谱说明了MAA与氧化乐果之间的结合作用,并利用扫描电子显微镜（SEM）观察了所制微球的外观形态和粒径分布。静态吸附实验证明MIPMs对氧化乐果的吸附量明显大于NMIPMs。Scatchard分析表明,MIPMs在识别氧化乐果过程中存在两类结合位点：高亲和位点的解离常数KD1=0.131mmol/L,最大表观结合量Qmax1=3.890μmol/g,低亲合位点解离常数为KD2=5.178mmol/L,最大表观结合量Qmax2=62.232μmol/g。选择性吸附实验结果表明MIPMs对氧化乐果的吸附量最大,具有很好的选择性能。
Molecularly imprinted microspheres（MIPMs） for recognition of omethoate and non-molecularly imprinted microspheres（NMIPMs） were synthesized by suspension polymerization.Methacrylic acid was used as functional monomer.Then,morphologies of MIPMs and NMIPMs were characterized by scanning electron microscopy（SEM）,which showed that the diameters were between 5-25μm.The isotherm binding of MIPMs and NMIPMs at different concentrations of omethoate demonstrated that MIPMs absorbed more substrates than NMIPMs.The data obtained in equilibrium adsorption experiment was processed by Scatchard analysis,which displayed that the MIPMs had two different affinity binding sites.The dissociation constants were calculated to be 0.131 and 5.178mmol/L,and the maximum absorption quantities were estimated to be 3.890 and 62.232μmol/g respectively.MIPMs also showed good selectivity for omethoate through the isotherm binding test of three other different substrates which were diomethoate,isocarbophos and phorate.
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