By using an LKB2277 BioActivity Monitor (heat conduction microcalorimeter),stopped-flow method,the thermogenetic curves of Escherichia coli growth at 37℃ inhibited by four kinds of heavy metal ions are determined,and parameters such as growth rate constants,inhibitory ratio,half-inhibitory concentration etc.were obtained.The experimental results show that heavy metal ions can inhibit Escherichia coli growth obviously,but low concentration of Cu2+ has a promoting action.The half inhibitory concentrations of Zn2+,Cd2+,Hg2+and Cu2+ are 28.3,10.9,3.19 and 69.6μg.mL-1,respectively.Judged from the rate constant,the half-inhibitory concentration and the molecular mass,the inhibitory sequence is Hg2+＞Cd2+＞Zn2+＞Cu2+.This microclorimetric bioassay for acute cellular toxicity is based on metabolic heat evolution from cultured cells.The assay is quantitative,inexpensive,and versatile;moreover,toxicological information can be obtained with cell from other species of interest.
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