获得高质量蛋白质晶体一直是X射线衍射解析蛋白质结构的瓶颈问题,因此研究提高蛋白质晶体质量的方法具有非常重要的意义.近年来人们从影响蛋白质结晶过程的各个因素出发,发展了一系列提高蛋白质晶体质量的方法,如在亚稳区生长蛋白质晶体、利用特殊环境影响蛋白质晶体生长的动力学过程、利用去垢剂提高蛋白质晶体质量和分子工程改造蛋白质分子提高晶体质量等.分析和评述了这些方法影响蛋白质结晶过程的机理.
Obtaining high quality crystal of protein has always been the bottleneck of structure determination by X-ray crystallography. In recent years, crystallographers have developed a series of method to improve the quality of protein crystals, such as crystal growth in metastable zone, influencing the kinetics process of protein crystal in special environment, obtaining high quality crystals by adding detergent and reductive methylation of proteins. In this paper,the principle of improving the protein crystal quality is discussed and reviewed.
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