考察了面包酵母发酵液直接催化4-氯-乙酰乙酸乙酯(COBE)的不对称还原反应,并进行了手性添加物的筛选和反应条件的优化实验. 结果表明,以β-环糊精为手性添加物时,酵母发酵液催化COBE不对称还原生成光活性产物(S)-4-氯-3-羟基-丁酸乙酯((S)-CHBE)的产率和ee值分别高达76%和92%. 在一定条件下,增大β-环糊精浓度,有利于(S)-CHBE的生成. 最佳酵母菌培养时间为16～18 h, 最佳反应温度和pH值分别为29～31 ℃和7.2.
参考文献
| [1] | Patel R N .[J].Advanced Synthesis and Catalysis,2001,343(6-7):527. |
| [2] | Kataoka M;Kita K;Wada M;Yasohara Y .[J].Applied Microbiology and Biotechnology,2003,62(5-6):437. |
| [3] | 朱文洲,许建和,俞俊棠.面包酵母催化乙酰乙酸乙酯的不对称还原反应[J].华东理工大学学报（自然科学版）,2000(02):154-156. |
| [4] | Yamada-Onodera K;Kawahara N;Tani Y;Yamamoto H .[J].Engineering in Life Sciences,2004,4(05):413. |
| [5] | 杨忠华,姚善泾.水相中酵母细胞催化4-氯乙酰乙酸乙酯不对称还原反应[J].催化学报,2004(06):434-438. |
| [6] | 欧志敏,吴坚平,杨立荣,岑沛霖.微生物法还原氯代苯乙酮制备手性醇[J].微生物学报,2003(04):523-526. |
| [7] | Kataoka M;Yamamoto K;Kawabata H;Wada M Kita K Yanase H Shimizu S .[J].Applied Microbiology and Biotechnology,1999,51(04):486. |
| [8] | 杨忠华,姚善泾.有效控制活性酵母细胞催化β-羰基酯不对称还原反应立体选择性的研究[J].催化学报,2004(10):805-808. |
| [9] | Hayakawa R;Shimizu M;Fujisawa T .[J].Tetrahedron,1997,8(19):3201. |
| [10] | Zhou B N;Gopalan A S;Van Middlesworth F;Shien W R Sih C J .[J].Journal of the American Chemical Society,1983,105(18):5925. |
| [11] | Nakamura K;Kawai Y;Oka S;Ohno A .[J].Bulletin of the Chemical Society of Japan,1989,62(03):875. |
| [12] | Vitinius U;Schaffner K;Demuth M .New strategies improve the efficiency of the baker's yeast reduction of ketoesters: near UV irradiation and a two-substrate application[J].Journal of Photochemistry and Photobiology, A. Chemistry,2005(2):197-210. |
| [13] | Yamamoto H;Matsuyama A;Kobayashi Y .[J].Applied Microbiology and Biotechnology,2003,61(02):133. |
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