主要探讨不同含氮量的无镍奥氏体不锈钢材料对血管内皮细胞及血小板粘附的影响。通过溶血率和血小板粘附试验考察不同含氮量的无镍奥氏体不锈钢材料的血液相容性;通过MTT试验和细胞粘附试验考察不同含氮量的无镍奥氏体不锈钢材料的细胞相容性。结果表明不同含氮量的无镍奥氏体不锈钢材料的溶血率都低于国家标准的5%,对血小板粘附的影响不显著;粘附在高氮无镍奥氏体不锈钢材料表面的血管内皮细胞数量均多于钛合金材料,且细胞生长状态良好;细胞毒性试验表明,不同含氮量的高氮无镍奥氏体不锈钢材料和对照组钛合金材料对血管内皮细胞没有产生明显的毒副作用。
Effects of high nitrogen nickel-free stainless steel on the adhesion of human umbilical vein endothelial cells（HUVECs） and blood platelets were investigated.The blood platelets adhesion test and blood hemolysis rate were used to evaluate the blood compatibility of high nitrogen nickel-free stainless steel.Human umbilical vein endothelial cells（HUVECs） were cultured in vitro,using MTT cytotoxicity test,cell adhesion test,to evaluate the cell compatibility of high nitrogen nickel-free stainless steel.Results showed that the hemolysis rate of high nitrogen nickel-free stainless steel was well below the accepted threshold value of 5%,but the effect on platelet adhesion was not obvious;the number of HUVECs adhesion on surface of high nitrogen nickel-free stainless steel were significantly more than that on the surface of titanium,and the cells in the surface showed a good growth situation.MTT test showed that both the high nitrogen nickel-free stainless steel and titanium alloy had no obvious effect on the HUVECs.
参考文献
| [1] | 白焱,牟春平,周珩,杨光远.冠状动脉介入治疗对冠心病患sICAM-1、MMPs-9的影响[J].中国伤残医学,2009(06):17-19. |
| [2] | Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood-contacting biomaterial[J].Journal of biomedical materials research, Part A,2010(2):341-349. |
| [3] | Luo Caifeng;Zheng Yiming;Diao Ziji et al.Review:research progress and future prospects for promoting endothelialization on endovascular stents an preventing restenosis[J].Journal of Medical and Biological Engineering,2011,31(05):307-316. |
| [4] | Mesenchymal stem cell seeding promotes reendothelialization of the endovascular stent[J].Journal of biomedical materials research, Part A,2011(3):442-449. |
| [5] | Meng Shen;Liu Zongjun;Shen Li et al.The effect of a layer-by-layer chitosan-heparin coating on the endothelialization and coagulation properties of a coronary stent systern[J].Biomaterials,2009,30(12):2276-2283. |
| [6] | Liu Q;Cheng XN;Fei HX .Effects of micro-magnetic field at the surface of 316L and NiTi alloy on blood compatibility.[J].Medical and Biological Engineering and Computing: Journal of the International Federation for Medical and Biological Engineering,2011(3):359-364. |
| [7] | 周星,梁建辉,朱明,张文娟,毛协民.人工食管用TiNi形状记忆合金的遗传毒性研究[J].功能材料,2009(03):443-445,454. |
| [8] | Jiang Tao;Wang Guixue;Qiu Juhui et al.Preparation and biocompatibility of polyvinyl alcohol small intestinal submucosa hydrogel membranes[J].Journal of Medical and Biological Engineering,2009,29(02):102-107. |
| [9] | 李超,李春江,蔡伟,赵连城,成艳,郑玉峰.PLGA携载紫杉醇涂层包被TiNi合金的表面特性与生物性能[J].功能材料,2005(05):750-752,756. |
| [10] | Haidopoulos M;Turgeon S;Sarra-Bournet C;Laroche G;Mantovani D .Development of an optimized electrochemical process for subsequent coating of 316 stainless steel for stent applications[J].Journal of Materials Science. Materials in Medicine,2006(7):647-657. |
| [11] | Sivakuma M;Rajeswari S .Investigation of failures in stainless steel orthopaedic implant devices[J].Journal of Materials Science Letters,1992,11(15):1039-1042. |
| [12] | Fidrhi J S;Eschbach L .Stainless steel in bone surgery injury[J].Injury.British Journal of Accident Surgery,2000,31(46):D2-D6. |
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