分别用吸附法、戊二醛(GA)和双醛淀粉(DAS)交联法将辣根过氧化物酶(HRP)固定在壳聚糖(Chitosan)上,通过对比3种固定化HRP的性能,选用Chitosan-DAS固定化HRP,其固定化酶相对活力和负载率分别为81％和68％.在Chitosan-DAS固定化HRP/H2O2酶促体系作用下制备香草醛-没食子酸共聚物,用FTIR、UV、NMR和GPC等对共聚物的结构进行了表征.将共聚物用作皮革鞣剂进行了应用实验,结果表明主鞣革收缩温度(Ts)为78.5℃,复鞣革平均增厚率为19.4％.
参考文献
| [1] | Shin-ichiro Tawaki;Yugo Uchida;Yasushi aeda;Isao Ikeda .HRP-catalyzed polymerization of sugar-based phenols in aqueous organic solvents[J].Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides,2005(1):71-74. |
| [2] | Lisa M. Colosi;Roger A. Pinto;Qingguo Huang;Walter J. Weber Jr. .Peroxidase-mediated Degradation Of Perfluorooctanoic Acid[J].Environmental Toxicology and Chemistry,2009(2):264-271. |
| [3] | Allen, BL;Kotchey, GP;Chen, YN;Yanamala, NVK;Klein-Seetharaman, J;Kagan, VE;Star, A .Mechanistic Investigations of Horseradish Peroxidase-Catalyzed Degradation of Single-Walled Carbon Nanotubes[J].Journal of the American Chemical Society,2009(47):17194-17205. |
| [4] | Pietro Vidossich;Giacomo Fiorin;Mercedes Alfonso-Prieto .On the Role of Water in Peroxidase Catalysis: A Theoretical Investigation of HRP Compound I Formation[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2010(15):5161-5169. |
| [5] | Sgalla S;Fabrizi G;Cacchi S;Macone A;Bonamore A;Boffi A .Horseradish peroxidase in ionic liquids - Reactions with water insoluble phenolic substrates[J].Journal of molecular catalysis, B. Enzymatic,2007(3/4):144-148. |
| [6] | Thaís E Schmidt;Luciano Caseli;David S. dos Santos Jr.;Osvaldo N. Oliveira Jr. .Enzyme activity of horseradish peroxidase immobilized in chitosan matrices in alternated layers[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2009(6):1889-1892. |
| [7] | Monier, M.;Ayad, D. M.;Wei, Y.;Sarhan, A. A. .Immobilization of horseradish peroxidase on modified chitosan beads[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,2010(3):324-330. |
| [8] | Tudorache M;Mahalu D;Teodorescu C et al.Biocatalytic microreactor incorporating HRP anchored on micro-/nano-lithographic patterns for flow oxidation of phenols[J].Journal of Molecular Catalysis B:Enzymatic,2011,69(3-4):133. |
| [9] | 孙建华,戴荣继,邓玉林.酶固定化技术研究进展[J].化工进展,2010(04):715-721. |
| [10] | 左鹏,于少明,杨杰茹,章璟嵩,张迟.辣根过氧化物酶固定化载体材料的研究进展[J].材料导报,2007(11):46-49. |
| [11] | 沈斌,魏荣卿,刘晓宁,朱建星,欧阳平凯.胺化聚苯乙烯载体柔性固定化木瓜蛋白酶[J].化工学报,2005(06):1070-1075. |
| [12] | 魏荣卿,沈斌,刘晓宁,汪海萍,王宇星,欧阳平凯.壳聚糖载体柔性固定化木瓜蛋白酶[J].过程工程学报,2005(02):183-187. |
| [13] | 于少明,程俊,左鹏,陈天虎,史铁钧.以层柱黏土为载体固定辣根过氧化物酶[J].化工学报,2006(12):3021-3024. |
| [14] | 刘飞,罗金岳.固体超强酸催化合成香草醛1,2-丙二醇缩醛[J].精细化工,2010(02):155-159. |
| [15] | 吴剑刚,孙建中,周其云.固定化HRP海藻酸钙/壳聚糖微球催化水溶性导电聚苯胺的合成与表征[J].华东理工大学学报（自然科学版）,2006(06):695-698. |
| [16] | Wu Yimin;Biswas N;Bewtra JK;Taylor KE .Kinetic Model for Removal of Phenol by Horseradish Peroxidase with PEG[J].Journal of Environmental Engineering,1999(5):0451-0458. |
| [17] | 李运,马建中,高党鸽,吕斌.新型醛鞣剂的制备及其应用[J].中国皮革,2008(09):43-46. |
| [18] | Entezari MH;Petrier C .A combination of ultrasound and oxidative enzyme: sono-biodegradation of phenol[J].Applied Catalysis, B. Environmental: An International Journal Devoted to Catalytic Science and Its Applications,2004(4):257-263. |
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