通过表面引发连续原子转移自由基聚合的方法在玻璃表面接枝了聚(N-异丙基丙烯酰胺)-b聚苯乙烯(PNIPAAm-b-PS)双嵌段聚合物刷.测试结果表明改性表面具有一定的浸润性温度响应性以及良好的生物学性能.与未改性的玻璃表面相比,改性后的表面具有良好的排斥血浆蛋白质的非特异性吸附能力.此外,PS链段的引入在不影响表面温敏性调控细胞黏附/脱附的务件下能够有效提高PNIPAAm改性表面在温度高于其低临界溶解温度(LCST)时对细胞的黏附能力.
参考文献
| [1] | Schild H G .Poly (N-isopropylacrylamide):Experiment,theory and application[J].Progress in Polymer Science,1992,17(02):163. |
| [2] | Ivaylo Dimitrov;Barbara Trzebicka;Axel H.E.Miiller .Thermosensitive water-soluble copolymers with doubly responsive reversibly interacting entities[J].Progress in Polymer Science,2007(11):1275-1343. |
| [3] | Okano T.;Okuhara M.;Sakai H.;Sakurai Y.;Yamada N. .MECHANISM OF CELL DETACHMENT FROM TEMPERATURE-MODULATED, HYDROPHILIC-HYDROPHOBIC POLYMER SURFACES[J].Biomaterials,1995(4):297-303. |
| [4] | Noriaki Matsuda;Tatsuya Shimizu;Masayuki Yamato;Teruo Okano .Tissue Engineering Based on Cell Sheet Technology[J].Advanced Materials,2007(20):3089-3099. |
| [5] | Akiyama Y;Kikuchi A;Yamato M;Okano T .Ultrathin poly(N-isopropylacrylamide) grafted layer on polystyrene surfaces for cell adhesion/detachment control[J].Langmuir: The ACS Journal of Surfaces and Colloids,2004(13):5506-5511. |
| [6] | Fukumori K;Akiyama Y;Yamato M .Temperature-responsive glass coverslips with an ultrathin poly(N-isopropylacrylamide) layer.[J].Acta biomaterialia,2009(1):470-476. |
| [7] | Mizutani A;Kikuchi A;Yamato M;Kanazawa H;Okano T .Preparation of thermoresponsive polymer brush surfaces and their interaction with cells.[J].Biomaterials,2008(13):2073-2081. |
| [8] | Li LH;Zhu Y;Li B;Gao CY .Fabrication of Thermoresponsive Polymer Gradients for Study of Cell Adhesion and Detachment[J].Langmuir: The ACS Journal of Surfaces and Colloids,2008(23):13632-13639. |
| [9] | Qian Yu;Yanxia Zhang;Hong Chen;Zhaoqiang Wu;He Huang;Chi Cheng .Protein adsorption on poly(N-isopropylacrylamide)-modified silicon surfaces: Effects of grafted layer thickness and protein size[J].Colloids and Surfaces, B. Biointerfaces,2010(2):468-474. |
| [10] | Chen H;Zhang Y;Li D et al.Surfaces having dual fibrinolyric and protein resistant properties by immobilization of lysine on polyurethane through a PEG spacer[J].Journal of Biomedical Materials Research Part A,2009,90(03):940. |
| [11] | Sun T L;Wang G J;Feng L et al.Reversible switching between superhydrophilicity and superhydrophobieity[J].Angewandte Chemie International Edition,2004,43(03):357. |
| [12] | Rowe MA;Hammer BAG;Boyes SG .Synthesis of surface-initiated stimuli-responsive diblock copolymer brushes utilizing a combination of ATRP and RAFT polymerization techniques[J].Macromolecules,2008(12):4147-4157. |
| [13] | Zhao, Bin;Brittain, William J. .Synthesis of tethered polystyrene-block-poly(methyl methacrylate) monolayer on a silicate substrate by sequential carbocationic polymerization and atom transfer radical polymerization[J].Journal of the American Chemical Society,1999(14):3557-0. |
| [14] | Gao X;Feng W;Zhu SP;Sheardown H;Brash JL .A facile method of forming nanoscale patterns on poly(ethylene glycol)-based surfaces by self-assembly of randomly grafted block copolymer brushes[J].Langmuir: The ACS Journal of Surfaces and Colloids,2008(15):8303-8308. |
| [15] | Prime K L;Whitesides G M .Adsorption of proteins onto surfaces containing end-attached oligo (ethylene oxide):A model system using self-assembled monolayers[J].Journal of the American Chemical Society,1993,115(23):10714. |
| [16] | da-Silva RM;Mano JF;Reis RL .Smart thermoresponsive coatings and surfaces for tissue engineering: switching cell-material boundaries.[J].Trends in biotechnology,2007(12):577-583. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%