采用乙二胺四乙酸二酐(EDTAD)和醛基封端聚乙二醇(PEG-dial)对明胶蛋白进行酰化及还原胺化改性,然后与戊二醛(GTA)交联制备了复合改性明胶蛋白凝胶.研究了EDTAD、PEG-dial、交联剂等的用量对凝胶性能的影响,结果表明,随EDTAD用量的增多及GTA用量的减少凝胶的平衡溶胀率增大,PEG-dial的引入提高了破裂强度,所得凝胶的溶胀率为160g/g,破裂强度为16g.采用IR和SEM对其进行结构表征,结果表明,改性后明胶蛋白上引入了羧基和长碳链,复合改性后凝胶的微观结构更有序.
A EDTAD-PEG-dial-gelatin compound modified gel is prepared firstly by acylating gelatin protein with ethylenediaminetetraacetic dianhydride(EDTAD),followed by reducing amination with PEG-dial,and then by cross-linking with glutaraldehyde.The effects of the amount of EDTAD,PEG-rdial and glutaraldehyde (GTA) on the properties of gelatin gel are discussed.The results show that the swelling ratio of gels increases with increasing amount of EDTAD or decreasing amount of GTA.The rupture strength of gels is improved by introducing PEG-dial into gelatin molecules.The swelling ratio and rupture strength of gels are 160g/g and 16g respectively.The acylated gelatin protein is characterized by IR and the gel is characterized by SEM,it is found that more hydrophilic carboxyl group and long carbon chain of PEG are introduced into gelatin molecules,and a more ordered structure is obtained by compound modifying.
参考文献
| [1] | Yu H Q;Xiao C B .Synthesis and properties of novel hydrogels from oxidized konjac glucomannan crosslinked gelatin for in vitro drug delivery[J].Carbohydrate Polymers,2008,72:479. |
| [2] | Weng L;Ivanova ND;Zakhaleva J;Chen W .In vitro and in vivo suppression of cellular activity by guanidinoethyl disulfide released from hydrogel microspheres composed of partially oxidized hyaluronan and gelatin.[J].Biomaterials,2008(31):4149-4156. |
| [3] | Yoshiaki Hirano;David J. Mooney .Peptide and Protein Presenting Materials for Tissue Engineering[J].Advanced Materials,2004(1):17-25. |
| [4] | Rowe SL;Lee S;Stegemann JP .Influence of thrombin concentration on the mechanical and morphological properties of cell-seeded fibrin hydrogels.[J].Acta biomaterialia,2007(1):59-67. |
| [5] | Chee-Yuen Gan;Lai-Hoong Cheng;Easa A. M. .Evaluation of microbial transglutaminase and ribose cross-linked soy protein isolate-based microcapsules containing fish oil.[J].Innovative Food Science & Emerging Technologies,2008(4):563-569. |
| [6] | 滕淑华,陈丽娟.明胶的基团改性及其对明胶性能的影响[J].明胶科学与技术,2003(01):1-7. |
| [7] | Harris J M;Struck E C;Case M G et al.Synthesis and characterization of poly (ethylene glycol) derivatives[J].Journal of Polymer Science Part A:Polymer Chemistry,1984,22:341. |
| [8] | 顾雪蓉;朱育平.凝胶化学[M].北京:化学工业出版社,2005:41. |
| [9] | Hwang DC;Damodaran S .CHEMICAL MODIFICATION STRATEGIES FOR SYNTHESIS OF PROTEIN-BASED HYDROGEL[J].Journal of Agricultural and Food Chemistry,1996(3):751-758. |
| [10] | Big A;Cojazzi G;Panzavolta S;Rubini K;Roveri N .Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking.[J].Biomaterials,2001(8):763-768. |
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