采用酸碱中和滴定法制备羟基磷灰石(HA)粉末,X射线衍射分析仪(XRD)检测其相组成,结果表明其具有较高的纯度和结晶程度.以该粉末为原料通过喷雾干燥法制备出粒径合适的羟基磷灰石微载体,采用扫描电镜观察其形貌和粒径分布,并通过原子吸收法检测微载体浸提液对钙离子浓度的影响,MTT法检测微载体浸提液对细胞毒性的影响,IPP软件分析计算浸提液对细胞铺展的影响,细胞计数法分析浸提液对细胞增殖的影响.结果表明,微载体首次浸提液中钙离子浓度、细胞的活性、细胞的铺展以及细胞的增殖与对照组相比均有显著差异,二次浸提液与对照组相比均无显著差异,即二次浸泡后的微载体具有较好的生物相容性.
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