用冷冻干燥法制备不同比例的纳米羟基磷灰石/壳聚糖-明胶(n-HA/CS-Gel)三维多孔支架材料,加入含骨形态发生蛋白-2(BMP-2)基因的质粒后再次冷冻干燥,制成具有基因缓释功能的组织工程支架材料.对支架材料的形貌结构、力学性能、体外降解特性、质粒的体外释放特性及质粒完整性进行检测.结果显示:纳米羟基磷灰石均匀分散在支架材料中,随着其含量的增加,孔隙率减少,抗压强度提高,在体外降解速度减慢.质粒DNA在体外1～3d快速释放,并能够维持释放5周以上,随着纳米羟基磷灰石含量的增加,释放时间延长,释放出的质粒DNA能够保持完整性.具有基因缓释功能的n-HA/CS-Gel支架材料是一种有望用于临床的新型骨组织工程支架材料.
Nano-hydroxyapatite/chitosan-gelatin network(n-HA/CS-Gel) scaffolds with different mass ratios of nano-hydroxyapatite were fabricated by freeze-drying. Plasmid DNA containing bone morphogenesis proteins-2 (BMP-2) was added into scaffolds and dried by freeze-drying. The morphology and structure, mechanical properties, in vitro degradation and DNA release kinetics of scaffolds were characterized. The results indicate that the addition of n-HA decreases slightly porosities of the scaffolds, while increases the compressive strength and steps down the degradation of the scaffolds in vitro. The scaffolds show rapid release of Plasmid DNA in 1-3 d and sustain release more than 5 weeks. The scaffolds has protective effects on the integrity of the plasmid DNA. The addition of n-HA steps down the release of plasmid DNA from scaffolds. The results suggest that the plasmid released n-HA/CS-Gel scaffold is a sort of hopeful candidate for bone tissue engineering.
参考文献
| [1] | Lim SH;Liao IC;Leong KW .Nonviral gene delivery from nonwoven fibrous scaffolds fabricated by interfacial complexation of polyelectrolytes.[J].Molecular therapy: the journal of the American Society of Gene Therapy,2006(6):1163-1172. |
| [2] | Huang YC;Simmons C;Kaigler D;Rice KG;Mooney DJ .Bone regeneration in a rat cranial defect with delivery of PEI-condensed plasmid DNA encoding for bone morphogenetic protein-4 (BMP-4).[J].Gene therapy,2005(5):418-426. |
| [3] | Zhang Y F;Cheng X R;Wang J W et al.[J].Biochemical and Biophysical Research Communications,2006,344(01):362. |
| [4] | Park S J;Kim C H .[J].J Tissue Eng Regen Med,2007,4(04):471. |
| [5] | Rohanizadeh R;Swain MV;Mason RS .Gelatin sponges (Gelfoam (R)) as a scaffold for osteoblasts[J].Journal of Materials Science. Materials in Medicine,2008(3):1173-1182. |
| [6] | Lin X Y;Fan H S;Li X D et al.[J].Key Engineering Materials,2005,284-286:553. |
| [7] | 赵峰,尹玉姬,宋雪峰,姚康德,郭善一,郭若霖,张镜宇.壳聚糖-明胶网络/羟基磷灰石复合材料支架的研究--制备及形貌[J].中国修复重建外科杂志,2001(05):276-279. |
| [8] | Liu X H;Ma P X .[J].Annals of Biomedical Engineering,2004,32(03):477. |
| [9] | Zhang Y;Zhang M Q .[J].Journal of Biomedical Materials Research,2001,55(03):304. |
| [10] | Huang Y C;Connell M;Park Y et al.[J].Journal of Biomedical Materials Research,2003,A 67:1384. |
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