使用冰滴为致孔剂制备表面大孔、内部孔洞相连的新型壳聚糖(HPCS)支架,将其与聚乳酸复合制备出三维蜂窝状孔洞结构的复合支架(THCP).对HPCS和THCP进行了表面形貌、力学性能、细胞相容性等方面的表征,并与常规冻干法所制备的壳聚糖(CS)海绵进行了对比.结果表明,在HPCS海绵表面均匀分布着大而开放的孔洞,大孔内部形成相互贯通的小孔,在THCP复合海绵内部形成了相互贯通的蜂窝状结构.THCP支架的力学强度比纯壳聚糖支架有明显的提高,其细胞活性及细胞增殖指数等都明显优于HPCS及CS海绵.因此,对壳聚糖拓扑结构的这种设计不仅弥补了常规冻干支架的缺陷,提高了细胞相容性,还将表面图案化改性应用延伸到了三维尺度.
参考文献
[1] | S.Pakakrong, S.Orawan, N.Artphop, C.Poonlarp,P.Prasit, S.Pitt, In vitro biocompatibility of electrospun and solvent-cast chitosan substrata towards Schwann,osteoblast,keratinocyte and fibroblast cells,European,Polymer Journal,3(46),428(2010) |
[2] | W.Teo,W.He,S.Ramakrishna,Electrospun scaffold tailored for tissue-specific extracellular matrix,Biotechnol.,1(9),918(2006) |
[3] | I.Kim,S.Seo,H.Moon,M.Yoo,Ⅰ.Park,B.Kim,C.Cho,Chitosan and its derivatives for tissue engineering applications,Biotechnol.Adv.,26(1),1(2008) |
[4] | A.Martino,M.Sittinger,M.Risbud,Chitosan:a versatile biopolymer for orthopaedic tissue-engineering,Biomaterials,26(30),5983(2005) |
[5] | E.Khor,L.Lim,Implantable applications of chitin and chitosan,Biomaterials,23(24),2339(2003) |
[6] | Y.Huang,M.Siewe,S.V.Madilhally,Effect of spatial architecture on cellular colonization,Biotechnol. Bioeng.,93(1),64(2006) |
[7] | H.Zhang,Ⅰ.Hussain,M.Brust,M.F.Butler,S.P.Rannard,Aligned two-and three-dimensional structures by directional freezing of polymers and nanoparticles,Cooper AI.Nat.Mater.,4(10),787(2005) |
[8] | G.Chen,T.Ushida,T.Tateishi,Scaffold design for tissue engineering,Macromol.Biosci.,2(2),67(2002) |
[9] | S.Rui,X.Rui,H.Ling,L.Ying,X.Qiao,The structure and properties of chitosan/polyethyleneglycol/silica ternary hybrid organic-inorganic films,Chinese Journal of Polymer Science,26(5),621(2008) |
[10] | K.Whang,E.Healy,D.R.Elenz,Engineering bone regeneration with bioabsorbable scaffolds with novel microarchitecture,Southern Biomedical Engineering ConferenceProceedings,143(1998) |
[11] | Tian Ye,Wang Yingjun,Zhou Changren,Preparation of regular micro-pits polylactide film via phase-separation and its cell affinity evaluation,Journal of Applied Polymer Science,116(5),3162(2010) |
[12] | Young-Gwang Ko, Naoki Kawazoe, Tetsuya Tateishi,Preparation of chitosan scaffolds with a hierarchical porous structure,Wiley InterScience,93(2),341(2010) |
[13] | Shuilin Wu, Xiangmei Liu, Tao Hu, Paul K.Chu,J.P.Y.Ho,Y.L.Chan,A biomimetic hierarchical scaffold:natural growth of nanotitanates on three-dimensional microporous Ti-based metals, Nano Letters, 8(11),3803(2008) |
[14] | Son JoonGon,Kim GeunHyung,Three-dimensional plotter technology for fabricating polymeric scaffolds with micro-grooved surfaces,Journal of Biomaterials Science,Polymer Edition,20(14),2089(2009) |
[15] | Jian Tana and W.Mark Saltzmana,Biomaterials with hierarchically defined micro-and nanoscale structure,Biomaterials,25(17),3593(2004) |
[16] | HUANG Zhihai,LI Jihong,LIN Pinghua,DONG Yinsheng,GUO Chao,SHENG Xiaobo,CHU Chenglin,Electrochemistry assisted depositing hydroxyapatite coating in chitosan porous scaffold,Acta Polym Sin,(3),273(2009)(黄志海,李纪宏,林萍华,董寅生,郭超,盛晓波,储成林,壳聚糖多孔支架电化学辅助沉积羟基磷灰石涂层研究,高分子学报,(3),273(2009) |
[17] | M.C.Wake,C.W.Patrick Jr,A.G.Mikos,Pore morphology effects on the fibrovascular tissue growth in porous polymer substrates,Cell Transplant,3(4),339(1994) |
[18] | S.Zhang,F.Gelain,X.Zhao,Designer self-assembling peptide nanofiber scaffolds for 3D tissue cell cultures,Seminars in Cancer Biology,145(3),239(2010) |
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