低温保护剂溶液结晶过程的显微实验研究

工程热物理学报 , 2001, 22(4): 481-484.

1.上海理工大学制冷与低温研究所,

2.上海理工大学制冷与低温研究所,

基金项目: 国家自然科学基金(59836240) 国家自然科学基金(50076028)

A MICROSCOPIC STUDY OF THE CRYSTALLIZATION IN CRYOPROTECTENT AGENTS

本研究利用低温显微实验台记录了异相成核时冰晶的生成和生长情况,利用获得的图像,分析了异相成核情况下冷却速率对冰晶生长速率及冰晶形状的影响,同时本文对存在大量晶胚情况下的冰晶生成和生长也作了详细的分析,指出冰晶的生长空间也是影响冰晶形状的一个不可忽视的因素.

[1]	华泽钊;任禾盛.低温生物医学技术[M].北京:科学出版社,1994
[2]	Lovelock J E .The Denaturation of Lipid Protein Complexes as a Cause of Damage of Freezing[M].London,1957,147:427-433.
[3]	MERYMAN H T .Modified Model for the Mechanism of Freezing Injury in Erythrocytes[J].Nature,1968,218:333-336.
[4]	Mazur P;Leibo S P;Chu E H Y .A Two Factor Hypothesis of Freezing Injury-Evidence from Chinese Hamster Tissue Culture Cells[J].Experimental Cell Research,1972,71:345-355.
[5]	Angell C A.A Comprehensive Treatise. Franks F Ed.,Supercooled Water in Water[M].New York:Plenum,1982
[6]	Diller K R;Cravalho E G .A Cryomicroscope for the Study of Freezing and Thawing Processes in Biological Cells[J].Cryobiology,1971,7:191-199.
[7]	Kober Ch;Scheiwe M W;Wollhover K .A Microscope for the Analysis of Solution Polarization during Freezing[J].Cryobiology,1984,21:68-80.
[8]	Schwartz G J;Diller K R .Design and Fabrication of a Simple. Versatile Cryomicroscopy Stage[J].Cryobiology,1982,19:529-538.
[9]	Steponkus P L;Dowgert M F;Ferguson J R et al.Cryomicroscopy of Isolated Plant Protoplasts[J].Cryobiology,1984,21
[10]	Steponkus P L;Stout D E;Wolfe J et al.Freezinginduced Electrical Transients and Cryoinjury[J].Cryo-Letters,1984,5:343-348.
[11]	邬申义;姚柯敏;华泽钊 .大型低温生物显微镜系统的研制[J].仪器仪表学报,1988,9(01):90-93.

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