Silicon （Si） incorporated porous TiO2 coating （Si-TiO2） prepared on titanium （Ti） by micro-arc oxidation （MAO） technique was demonstrated to be cytocompatible in previous studies. In view of the potential clinical applications, a detailed in vitro study of the biological activity of Si-Ti02 coating, in terms of osteoblast （MC3T3-EI cells） morphology, proliferation, differentiation and mineralization was performed. Immunofluo- rescent staining indicated that cells seeded on the Si-TiO2 coating showed improved adhesion with developing mature cytoskeletons, which contained numerous distinct and well-defined actin stress fibers in the cell mem- branes compared with those on the Ti02 coating and Ti plate. Results from proliferation assay showed that the proliferation rate of cells seeded on the Si-TiO2coating was significantly faster than that on the TiO2 coating and Ti plate. Furthermore, the analysis of osteogenic gene expression demonstrated that the Si-Ti02 coating stimulated the expression of osteoblast-related genes and promoted differentiation and mineralization of MC3T3-EI cells. In addition, the Si-TiO2 coating differentially regulated Wnt signaling pathway by up-regulating the expression of low-density lipoprotein （LDL） receptor-related protein 5 （LrpS）, and downregulating the expression of Dickkopf-1 （Dkkl）. All together, these results indicate that the investigated titanium with Si-TiO2 coating is biocompatible and a good candidate material used as implants.
参考文献
| [1] | H. Ishizawa;M. Ogino .Hydrothermal precipitation of hydroxyapatite on anodic titanium oxide films containing Ca and p[J].Journal of Materials Science,1999(23):5893-5898. |
| [2] | C. APARICIO;F. J. GIL;J. A. PLANELL;E. ENGEL .Human-osteoblast proliferation and differentiation on grit-blasted and bioactive titanium for dental applications[J].Journal of Materials Science. Materials in Medicine,2002(12):1105-1111. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%