为了研究骨髓基质细胞转化的成骨细胞在碱热处理的涂层磷灰石钛表面的分化增殖,将钛片经过碱热处理后,浸泡于与类似体液的环境进行表面磷灰石涂层.应用骨髓成骨细胞作为实验工具,观察体外细胞在处理的涂层钛片上的生长,碱性磷酸酶以及降钙素的分泌.在早期,有磷灰石涂层的钛片成骨细胞黏附高于对照组;在晚期,两组之间没有明显差异;成骨细胞分化的标志--碱性磷酸酶活性和降钙素的表达,磷灰石涂层组都明显高于对照组.研究表明:碱热处理的磷灰石涂层钛能够促进成骨细胞黏附,有利于细胞向成骨分化.
Osteoblast differentiation of rabbit bone marrow cells on apatite-coated titanium by alkali-heat treatment was investigated in present paper. Titanium was subjected to alkali-heat treatment and soaked in acellular simulated body fluid to prepare surface apatite layer. Cell behavior and alkaline phosphatase activity and osteocalcin on apatite-coated titanium were compared with those on commercially pure titanium in vitro. The results show that initial cell adhesion on apatite-coated titanium is higher than that on pure titanium while cell growth rates at a late stage are not significantly different for both groups. Early and later osteoblast differentiation marks, alkaline phosphatase activity and osteocalcin, on apatite-coated titanium are higher than those on pure titanium. Apatite-coated titanium by alkali-heat treatment can increase osteoblast adhesion and displays a higher differentiation capacity for osteoblasts differentiation than that for commercially pure titanium.
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