利用30,90,180 Gy 3种剂量的12C6+重离子束辐照大葱种子,研究其在细胞水平和农艺性状的诱变效应并进行RAPD分析.通过与M1代的研究结果比较后表明:经过不同剂量12C6+重离子照射后能有效地诱导大葱细胞形成微核和染色体畸变,这种诱变效应,在M2代仍然有所表现.M1代大葱结果期的株高、白长、花序直径和种子产量随辐照剂量增加产生明显差别,其中30Gy辐照组增幅最大.大葱总水溶性蛋白质和维生素C的含量在30 Gy组中积累最多,在90 Gy组有明显下降.与M1代一致,M2代中大葱染色体微核率及RAPD分析所得的DNA多态性比率仍然与辐照剂量呈正相关,但比率整体下降;说明高能量重离子辐照造成的DNA变异在M2代被修复和淘汰.
参考文献
| [1] | Hirono Y,Smith H H,Lyman J T,et al.Rad Res,1970,44:204. |
| [2] | Hou Suiwen,Wu Dali,Zhang Yingcong,et al.J Radiat Res Radiat Process,2008,(4):78(in Chinese).(侯岁稳,吴大利,张颖聪,等.辐射研究与辐射工艺学报,2008,(4):78.) |
| [3] | Wang Ruixiang.Journal of Shanxi Teacher's University(Natural Science Edition),1998,(12):50(in Chinese).(王瑞祥.山西师范大学学报(自然科学版),1998,(12):50.) |
| [4] | Li Xianggao,Qi Zhangnian,Chen Mei,et al.Space Medicine&Medical Engineering,1996,(12):417(in Chinese).(李向高,祁章年,陈湄,等.航天医学与医学工程,1996,(12):417.) |
| [5] | Wu Zhenxiang,Li Sumei,Yin Guoxiang,et al.Journal of Anhui Agricultural University,1994,21(3):321(in Chinese).(邬振祥,李素梅,尹国香,等.安徽农业大学学报,1994,21(3):321.) |
| [6] | Zhang Zhihong,Du Liqun,Li Hongjie,et al.Acta Biophysica Sinica,1998,14(4):762(in Chinese).(张志宏,杜立群,李洪杰,等.生物物理学报,1998,14(4):762.) |
| [7] | Cheng Beijiu,Tian Qiuyuan,Lin Yi,et al.Journal of Anhui Agricultural University,1991,18(2):148(in Chinese).(程备久,田秋元,林毅,等.安徽农学院学报,1991,18(2):148.) |
| [8] | Li Xiaoguo,Zhang Hongwei.Xinjiang Agricultural Sciences-2002,39(4):238(in Chinese).(李小国,张宏伟.新疆农业科学,2002,39(4):238.) |
| [9] | Liang Qianjin,Hu Yulian,Zhang Genfa.Acta Biophysica Sinica,2002,18(2):251(in Chinese).(梁前进,胡玉莲,张根发.生物物理学报,2002,18(2):251.) |
| [10] | Xu Jingjing,Li Jing,Feng Xihuan,et al.Chinese Agricultural Science Bulletin,2008,24(11):307(in Chinese).(徐敬敬,李静,冯希环,等.中国农学通报,2008,24(11):307.) |
| [11] | Su Hua.Comparison and Appraisal on Major Characters of Welshi Onion Varieties Introduced from Japan(doctor thesis).Jinan:College of Horticultural Science and Engineeing,Shandong Agricultural University,2006,6-21(in Chinese).(苏华.日本大葱品种主要性状的比较鉴定(博士论文).济南:山东农业大学囝艺科学与工程学院,2006,6-21.) |
| [12] | Fan Zhicheng,Gao Zhaobo,Li Jianyou.China Vegetables,2004,(6):38(in Chinese).(樊治成,高兆波,李建友.中国蔬菜,2004,(6):38.) |
| [13] | Hou Suiwen,Qian Pingping,Guan Liping,et al.Journal of Nuclear Agricultural Sciences,2008,22(1):9(in Chinese).(侯岁稳,钱平平,管利萍,等.核农学报,2008,22(1):9.) |
| [14] | Zou Qi.The Plant-physiology Experiment Instructs.BeiJing:Chinese Agricuhure Publishing House,2006,129-171(in Chinese).(邹琦.植物生理学实验指导.北京:中国农业出版社,2006,129-171.) |
| [15] | Chen Xuejun,Li Wenjian,Chen Jing,et al.Nuclear Physics Review,2008,25(2):176(in Chinese).(陈学君,李文建,陈婧,等.原子核物理评论,2008,25(2):176.) |
| [16] | Qian Pingping,Hou Suiwen,Wu Dali,et al.J Radiat Res Radiat Process,2007,25(4):212(in Chinese).(钱平平,侯岁稳,吴大利,等.辐射研究与辐射工艺学报,2007,25(4):212.) |
| [17] | Wei Zengquan,Xie Hongmei,Liang Jianping,et al.Nuclear Physics Review,2003,20(1):38(in Chinese).(卫增泉,颉红梅,梁剑平,等.原子核物理评论,2003,20(3):39.) |
| [18] | Zhang Jinlian,Hu Yue,Wei Zengquan,et al.Gansu Agriculture Science and Technology,1995,(2):2(in Chinese).(张金莲,胡越,卫增泉,等.甘肃农业科技,1995,(2):2.) |
| [19] | Li Guicheng,Wang Linhui,Luo Hongbing.Journal of Hunan Agricultural University(Natural Sciences),2007,33(10):558(in Chinese).(李贵成,王林辉,罗红兵.湖南农业大学学报(自然科学版),2007,33(10):558.) |
| [20] | Zhuang Chuxiong,Hu Weimin,Mei Mantong.Acta Agriculturae Nucleatae Sinica,1997,11(2):77(in Chinese).(庄楚雄,胡维民,梅曼彤.核农学报,1997,11(2):77.) |
| [21] | Liu Zhifang,Shao Junming,Tang Zhangxiong,et al.Journal of Nuclear Agricultural Sciences,2006,20(1):1(in Chinese).(刘志芳,邵俊明,唐掌雄,等.核农学报,2006,20(1):1.) |
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