研究了氨基葡萄糖（D-glucosamine,Glc-NH2）,N-乙酰氨基葡萄糖（N-acetyl-D-glucosamine,GlcNAc）和小分子壳聚糖（chitosan,CS）对酒精（C2H5OH）或四氯化碳（CCl4）诱导的小鼠肝细胞损伤的预防和修复作用。采用组织块培养法获取小鼠肝细胞,通过对小鼠传代培养过程中肝细胞活性的测定,证实该细胞在第6d达到最佳生长状态。分别用无糖培养基,含500或1000μg/mL GlcNH2、GlcNAc或小分子CS的培养基对肝细胞培养3d后用C2H5OH或CCl4诱导损害不同的时间,继续培养6d。结果发现GlcNH2、GlcNAc和小分子CS都能预防CCl4诱导的肝细胞损伤;而只有小分子CS对C2H5OH诱导的肝细胞损伤具有较好的预防效果。2000μg/mL的Glc-NH2、GlcNAc和小分子量CS对C2H5OH诱导损伤小鼠肝细胞的修复正相性都很明显;而对于CCl4诱导的肝细胞损伤,只有GlcNH2对CCl4诱导损伤30min的肝细胞具有修复作用。
The protective effects of D-glucosamine（GlcNH2）,N-acetyl-D-glucosamine（GlcNAc） and low molecular chitosan（CS） on C2H5OH-induced or CCl4-induced mouse hepatocytes damage were investigated in the present study.The mouse hepatocytes were obtained through tissue culture.The cells activity was investigated during the subculture and the results showed the mouse hepatocytes had optimal growth condition at 6d culture.The hepatocytes were exposed to C2H5OH or CCl4 for different times after 3d culture of sugar-free medium,500 or 1000μg/mL of GlcNH2,GlcNAc or low molecular CS medium,and the MTT assay was measured after 6d culture.The results indicated the GlcNH2,GlcNAc and low molecular CS all had preventive effect to CCl4-induced damage and only the low molecular CS had preventive effect to the C2H5OH-induced hepatocytes damage.2000μg/mL of GlcNH2,GlcNAc and low molecular CS all had positively effects on C2H5OH-induced hepatocytes damage recovery,indicating the potential applications in the treatment of C2H5OH-induced liver disease.
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