生物材料可通过表面改性引入活性基团来增强其生物相容性.采用紫外辐照方法,以亚铁离子辅助引发接枝聚合,将羧基引入聚酰胺66(PA66)膜表面,再用水溶性碳化二亚胺(WSC)作为缩合剂,进一步将明胶固定在聚酰胺膜表面,最终获得大分子修饰的聚酰胺材料.利用衰减全反射-傅里叶红外光谱(ATR-FTIR)、X射线光电子能谱(XPS)、扫描电子显微镜(SEM)和水接触角测量(WCA)等测试手段对PA66膜和表面改性PA66膜进行了表征.结果表明,在40℃条件下,光氧化的最佳时间为60min.接枝聚合后,膜的亲水性增强,水接触角由纯聚酰胺的67.5°变为固定明胶分子后的60°,并且膜表面的粗糙度增加.表面改性PA66膜可促进细胞活性,适用于组织工程中.
Surface modification is an applicable method to introduce bioactive species onto biomaterial surface for enhancing the biocompatibility. In this study,the carboxyl was introduced on the polyamide66 membrane surface by UV irradiation under induction by Fe~(2+) reduction. Gelatin was covalently immobilized onto the surface of polyamide66 (PA66) membrane using water-soluble carbodiimide (WSC) as the condensing agent. The properties of the pure and modified PA membrane were characterized by means of ATR-FTIR,XPS,SEM and water contact angle measurement. The results showed that the optimal reaction time was 60min in the photo-oxidation process at 40℃;. The water contact angle changed from the original value of 67. 5-60°for gelatin immobilized polyamide 66,which indicated the hydrophilicity of modified membranes was improved. Simultaneously,the modified polyamide66 surface exhibited an increase in roughness. The modified surface of PA66 membrane may promote cell activity in tissue engineering application.
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