本文建立了基于磁性微球表面氨基的荧光胺定量检测方法,通过研究荧光胺与磁性微球表面氨基反应的光学性质,优化了影响检测反应的条件。碱性条件下微球表面改性氨基硅烷在反应60h后基本可从微球表面完全解离下来,荧光胺分子与解离后的游离氨基反应20min即可得到稳定的荧光信号。我们发现缓冲溶液的pH值在8.0时所得到的荧光测试信号较稳定。所建立的方法操作简便快捷,结果的准确性较高。
Fluorescamine quantitative detection method for detecting the density of surface amino groups of magnetic microspheres was established and the reaction conditions were optimized via the investigation of the optical reaction properties between fluorescamine and amino groups.It is found that the modified aminosilane carrying amino groups will be completely dissociated from the surfaces of microspheres after 60h under alkaline conditions,which renders a stable fluorescent signal after a further reaction of 20min with fluorescamine.It is shown that the fluorescent signal is most stable with pH of 8.0 in the reaction system.The detection method of the density of amino groups on magnetic microspheres based on fluorescamine is simple and fast with a high accuracy.
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