磷酸钙陶瓷在非骨组织中构建组织工程骨研究

稀有金属材料与工程, 2009, 38(z2): 798-801.

磷酸钙陶瓷在非骨组织中构建组织工程骨研究

姚金凤 ^1, , 李晓宇 ^2, , 包崇云 ^3, , 范红松 ^4, , 张兴栋 ^5, , 陈治清 ^6,

1.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

2.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

3.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

4.四川大学,国家生物材料研究中心,四川,成都,610065

5.四川大学,国家生物材料研究中心,四川,成都,610065

6.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

基金项目: 国家自然科学基金(30672337) NCET(06-0782)

关键词：骨诱导性磷酸钙陶瓷 , 体内组织工程骨 , 非骨组织

A Study of Bone Graft with Calcium Phosphate Ceramics in Non-Osseous Tissue

Yao Jinfeng ^1, , Li Xiaoyu ^2, , Bao Chongyun ^3, , Fan Hongsong ^4, , Zhang Xingdong ^5, , Chen Zhiqing ^6,

1.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

2.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

3.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

4.四川大学,国家生物材料研究中心,四川,成都,610065

5.四川大学,国家生物材料研究中心,四川,成都,610065

6.四川大学,口腔疾病治疗国家重点实验室,四川,成都,610041

Keywords： osteoinductive calcium phosphate ceramic , in vivo tissue engineering bone , non-osseous tissue

采用体内骨组织工程的方法,探索骨诱导性磷酸钙陶瓷支架在不同非骨组织中构建骨移植物的可行性,并比较其差异,为体内骨组织工程的临床构建技术提供理论依据.方法为选取家犬的背部肌肉组织和脂肪组织为构建区,分别植入骨诱导性磷酸钙陶瓷支架以构建体内组织工程骨移植物.于术后4,6,12,24 w取样进行单光子计算机断层扫描(SPECT)、组织学检测,观察其构建过程,比较各个观测时间内,不同构建区的骨移植物中新骨组织的形成情况,评价不同非骨构建区域对体内骨组织骨移植物形成的影响.结果显示,骨诱导性磷酸钙陶瓷支架在肌肉组织和脂肪两处非骨组织中均可形成体内组织工程骨移植物.在构建初期,肌肉组中新骨形成的时间比脂肪组早,骨量也较多.但在构建24 w后,两组的新生骨量没有差异.较肌肉组织而言,在脂肪组织中构建体内组织工程骨移植物更有临床应用前景.

The study is aimed to compare the bone graft with osteoinductive calcium phosphate ceramics implanted in muscle and subcutaneous fat, and provide applying technique for the in vivo bone tissue engineering. Osteoinductive calcium phosphate ceramics columns with Φ5 mm×8 mm were prepared, and eight dogs were used in this experiment. Sixteen samples were implanted in each dog. Eight in dorsal muscles while the others in subcutaneous fat. The specimens were harvested at 4, 6, 12 and 24 w post operation, for gross observation, SPECT and histological studies. The osseous tissue formation at different time in the two non-osseous sites was compared and the new bone grafts in subcutaneous fat and intramuscular implantations were evaluated. The results demonstrated that the bone graft could be constructed not only in muscle but also in subcutaneous fat. 24 w after implantation, the amount of newly formed bone in the two non-osseous sites had no statistic difference. Compared to that in muscle, constructing bone graft in subcutaneous fat could have brighter prospect to clinical application.

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