定向诱导基因组局部突变技术（Targeting Induced Local Lesions IN Genomes,简称TILLINC）是一种全新的、高通量和低成本反向遗传学研究方法。近年来,随着突变筛选技术的革新,TILLING技术平台日趋多元化,使得TILLING技术的操作更为简单、快速,并广泛应用于作物育种研究领域。简要介绍了TILLING技术平台的最新发展动态,并初步探讨了将辐射诱变处理与TILLING高通量筛选相结合在诱变育种中的应用前景。
TILLING（Targeting Induced Local Lesions IN Genomes） is a novel,high-throughput and low-cost reverse genetics technique.In recent years,with innovation of the mutation screening techniques,TILLING platform has become more diversified,which makes the operation of TILLING technique more simple and rapid.For this reason,it is widely used in crop breeding research.In this paper,we summarized the latest progress of TILLING technique,meanwhile,we also discussed the prospect of combining irradiation mutation with the high-throughput TILLING technique in mutation breeding.
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