采用真空铸造技术制备了Mg-2Zn-0.2Mn三元镁合金,并对该合金体外生物生物相容性能进行了研究.采用血小板粘附、溶血率评价镁合金的血液相容性,采用细胞增殖实验(CCK-8)、细胞凋亡实验(AO/PI)评价镁合金的细胞相容性.结果表明:血小板粘附实验表明,与常用的AZ31B镁合金和316L不锈钢样品相比较,Mg-2Zn0.2Mn镁合金表面粘附的血小板数量少,且激活和团聚较轻微；溶血实验的结果表明Mg-2Zn0.2Mn的溶血率为4.7％,远小于AZ31B的80.9％溶血率.血小板黏附和溶血实验说明Mg-2Zn0.2Mn镁合金具有优异的血液相容性.细胞增殖和凋亡实验结果也表明,较AZ31B样品,Mg-2Zn0.2Mn样品表面显示了更好的内皮细胞粘附和增殖能力,说明Mg-2Zn0.2Mn镁合金具有很好的血液相容性和内皮细胞相容性,有望作为今后可降解血管支架的候选材料.
参考文献
| [1] | 张朝勇.生物可吸收镁合金药物支架研究进展[J].广西医科大学学报,2012(03):483-485. |
| [2] | Moravej M;Mantovani D .Biodegradable Metals for Cardiovascular Stent Application:Interests and New Opportunities[J].INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(07):4250-4270. |
| [3] | Mani G;Feldman MD;Patel D;Agrawal CM .Coronary stents: A materials perspective[J].Biomaterials,2007(9):1689-1710. |
| [4] | Erne P;Schier M;Resink TJ .The road to bioabsorbable stents:Reaching clinical reality[J].Cardiovascular and Interventional Radiology,2006,29(01):11-16. |
| [5] | Yeoheung Yun;Zhongyun Dong;Namheon Lee;Yijun Liu;Dingchuan Xue;Xuefei Guo;Julia Kuhlmann;Amos Doepke;H. Brian Halsall;William Heineman;Surya Sundaramurthy;Mark J. Schulz;Zhangzhang Yin;Vesselin Shanov;Douglas Hurd;Peter Nagy .Revolutionizing biodegradable metals[J].Materials Today,2009(10):22-32. |
| [6] | Juan W;Yonghui H;Manfred F.Maitz.A surfaceeroding poly (1,3-trimethylene carbonate) coating for fullybiodegradable magnesium-based stents:improving biodegradation behavior and biocompatibility[J].Acta Biomaterials |
| [7] | Hansi C;Arab A;Rzany A;Ahrens I;Bode C;Hehrlein C .Differences of platelet adhesion and thrombus activation on amorphous silicon carbide, magnesium alloy, stainless steel, and cobalt chromium stent surfaces.[J].Catheterization and cardiovascular interventions: Official journal of the Society for Cardiac Angiography & Interventions,2009(4):488-496. |
| [8] | Di Mario C;Griffiths H;Goktekin O;Peeters N;Verbist J;Bosiers M;Deloose K;Heublein B;Rohde R;Kasese V;Ilsley C;Erbel R .Drug-eluting bioabsorbable magnesium stent.[J].Journal of interventional cardiology,2004(6):391-395. |
| [9] | Heublein B;Rohde R;Kaese V et al.Biocorrosion of magnesium alloys:a new principle in cardiovascular implant technology[J].Heart,2003,89(06):651-656. |
| [10] | 陈先华,耿玉晓,刘娟.镁及镁合金功能材料的研究进展[J].材料科学与工程学报,2013(01):148-152. |
| [11] | Goodman SL;Grasel TG;Cooper SL;Albrecht RM .Platelet shape change and cytoskeletal reorganization on polyurethaneureas[J].Journal of Biomedical Materials Research,1989,23(01):105-123. |
| [12] | Hayash K;Kawamura Y .Structure and Mechanical Properties of Rapidly Solidified Ma-X Alloys[J].Materials Science Forum,2000,350:117-122. |
| [13] | Gooden D L .Hydro Magnesium Corrosion and Finishing of Magnesium Alloys[J].Gneneral Corrosion,1997,11:1-3. |
| [14] | 李冠群,吴国华,樊昱,丁文江.主要合金元素对镁合金组织及耐蚀性能的影响[J].铸造技术,2006(01):79-83. |
| [15] | McEver RP;Martin MN .A monoclonal antibody to a membrane glycoprotein binds only to activated platelets[J].Journal of Biological Chemistry,1984,259(15):9799-9804. |
| [16] | 耿丽彦,宋义全,张永虎.Zn含量对生物医用镁合金耐Hank，s模拟体液腐蚀的影响[J].材料保护,2012(08):28-30. |
| [17] | 陶海荣,张岩,何耀华,蒋垚,张绍翔,张小农.镁锌合金在动物体内降解及其相容性[J].中国组织工程研究与临床康复,2009(12):2232-2236. |
| [18] | Ward CA;Stanga RD;Zingg W;Herbert MA .Relation of proteins,platelets,and gas nuclei in adhesion to synthetic materials[J].American Journal of Physiology,1977,233(01):100-105. |
| [19] | ISO/TR 7405～1984.Biological Evaluation of Dental Materials Contents,Heamolysis Test (In Vitro Test Directly on Materials)[S]. |
| [20] | Yun, Y.;Dong, Z.;Tan, Z.;Schulz, M.J. .Development of an electrode cell impedance method to measure osteoblast cell activity in magnesium-conditioned media[J].Analytical and bioanalytical chemistry,2010(8):3009-3015. |
| [21] | Anneke Loos;Roland Rohde;Axel Haverich;Sabine Barlach .In Vitro and In Vivo Biocompatibility Testing of Absorbable Metal Stents[J].Macromolecular symposia,2007(0):103-108. |
| [22] | 杨柯,谭丽丽,任伊宾,张炳春,张广道,艾红军.AZ31镁合金的生物降解行为研究[J].中国材料进展,2009(02):26-30. |
| [23] | 金琪琳,黄云超.生物材料对细胞生物学行为的影响[J].材料科学与工程学报,2007(02):318-320,324. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%