由江汉油田采油厂污水中分离、提纯出来的硫酸盐还原菌（SRB）菌株，采用APIRP-38推荐使用的培养基生成生物膜。利用交流阻抗技术研究了生物膜与腐蚀之间的关系，细菌新陈代谢产物及腐蚀产物的分析借助于电子探针用气相色谱/质谱联用技术（GC/MS）。结果表明，SRB能够达到电极表面，且在其表面形成腐蚀产物的细菌生物膜混合层，细菌包裹在腐蚀主物中，形成局部微生镜。GC/MS及电子探针的研究表明，在此微生镜中细菌新陈代谢有脂肪酸、磷铁化合物、硫铁化合物生成。生物膜的存在为SRB对胞外物质的富集提供了良好的场所，且由于代谢产物造成的微生镜的差异，促进了点蚀的形成。
In this paper,the biofilm of sulfate reducing bacteria(SRB)which plays an important role in corrosion of carbon steel under anaerobic condition has been studied.The postgate medium was used to culture Jianghan oilfield SBR.Electrochemical impedance spectroscopy(EIS)was used to analyse the electrode process of carbon steel with SBR biofilm.The advantage of EIS lies in its minor disturbance of surface effect of the biofilm.X-ray diffraction and gas chromatography and mass spectrometry(GC/MS)techniques were used to analyse the corrosion products and the metabolites of SRB.The results showed that the main metablites of SRB sampled from Jianghan oilfield were butanic and benzoic acid and the corrosion products contained FeS and Fe2P. The variation of activit of the SRB biofilm with SRB growth can be found from the EIS in SRB-inoculation media.The extracellular polymeric substance of biofilm stimulated the concentration of nutrition and corrosion products,so it could accelerate the corrosion process.
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