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本文以红细胞悬液为研究模型,通过在对其进行慢速冻结的过程中引入静电场,具体研究了静电场对降温过程中红细胞悬液冻结特性的影响.实验结果表明:静电场在一定程度上改变了冰晶的形成与生长特性,抑制了晶核的形成,使晶枝出现不对称生长,并减缓了冰晶的生长速度,这些影响随着场强的增加而逐渐加强.在较强的静电场的影响下,冰晶明显变粗,最终成为块状,细胞也不再与冰晶分离而是完全融入粗大的冰晶之中,在冻结的末期,细胞不再受到冰晶挤压,从而减少了其所受的机械性损伤.

参考文献

[1] Mazur P;Leibo S P;Chu E H Y .A Two Factor Hypothesis of Freezing Injury-Evidence from Chinese Hamaster Tissue Culture Cells[J].Experimental Cell Research,1972,71:345-355.
[2] Nei T .Mechanism of Hemolysis of Erythrocytes by Freezing at Near-Zero Temperatures,I.Microscopic Observation of Hemolyzing Erythrocytes During the Freezing and Thawing Process[J].Cryobiology,1967,4:153-156.
[3] Bronstein V L;Itkin Y A;Ishkov G S .Rejection and Capture of Cells by Ice Crystals on Freezing Aqueous solutions[J].Journal of Crystal Growth,1981,52:345-349.
[4] Ishiguro H;Rubinsky B .Mechanical Interactions Between Ice Crystals and Red Blood Cells During Directional Solidification[J].Cryobiology,1994,31:483-500.
[5] Le Ren Tao;Tse Chao Hua .Microscopic Study of Crystal Growth in Cryo-Preservation Solution[J].Annals of The New York Academy of Sciences,2002,972:151-157.
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