首先制备壳聚糖的衍生物羧甲基壳聚糖（carboxymethyl chitosan,CMCS）,然后以薄膜水化法制备VE脂质体,用CMCS对VE脂质体进行包覆,制备CMCS包覆VE脂质体。形态学观察表明CMCS成功地包覆在VE脂质体的表面,CMCS包覆VE脂质体呈球形或近球形,分散性良好。CMCS的浓度对CMCS包覆VE脂质体的Zeta电位和平均粒径影响不大。随着CMCS浓度的升高,CMCS包覆VE脂质体的保留率升高,沉降率降低,结果表明CMCS的包覆有效提高了VE脂质体的储存稳定性。
In the present work,we report the synthesis of carboxymethyl chitosan（CMCS） and the preparation of CMCS-coated VE liposome.TEM images of the CMCS-coated VE liposome showed that the particles were spherical and well separated.The influences of the CMCS concentration were not obvious to the zeta potential and average particle size of the CMCS-coated VE liposome.The conserving rate of the liposome increased and sedimentation volume rate decreased with the increasing CMCS concentration.The results indicated that the coating of the CMCS enhanced the storage stability of the VE liposome effectively.
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