通过3种不同浓度的不锈钢浸提液处理体外培养的小鼠成纤维细胞L929, 采用流式细胞术PI染色法检测L929细胞的凋亡率及细胞周期分布情况, 并用电镜观察细胞凋亡的形态. 同时应用异硫氰酸荧光素标记的膜联蛋白结合碘化丙啶染色双参数技术对细胞死亡类型进行定量检测. 与Tilite含钛医用合金、Co-Cr合金相比较的结果表明,26-1超纯铁素体不锈钢是以时间依赖的方式诱导L929细胞凋亡, 凋亡率与浸提液呈浓度依赖关系. 26-1超纯铁素体不锈钢符合临床应用材料的生物相容性要求, 可以用于对磁性附着体的外包裹并可安全地应用于临床.
The magnetic attachment wrapped with 26-1 ultra-purified ferrite stainless steel can improve its corrosion resistance. The fibroblast L929 cells of mouse were treated in vitro through 3 kinds of leaching liquors of stainless steel to detect the apoptosis rate and cell cycle distribution of L929 by the flow cytometry PI staining method, and the morphology of cell apoptosis was observed by electron microscope. Compared with the Tilite alloy and Co-Cr alloy, the type of cell apoptosis was quantitatively determined by di-parameter technique in which the annexin was marked by the fluorescein isothiocyanate (FIT) and stained by the iodonadine. The result showed that 26-1 ferrite stainless steel can induce the apoptosis of L929 cells with a fashion of time-dependence, and the apoptosis rate depends on the concentration of the leaching liquor. It also proved that 26-1 ferrite stainless steel meets the biocompatibility as a clinical material and can be safely applied in clinic.
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