{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":4,"startPagecode":1},"records":[{"abstractinfo":"复杂肽段混合物的有效分离是高覆盖率地鉴定蛋白质混合物的前提.\"鸟枪法\"(Shotgun)蛋白质组学研究策略通常采用蛋白酶切、二维液相色谱-串联质谱分析肽段混合物从而鉴定蛋白质,其中高效率地分离肽段混合物是关键步骤之一.本文通过pH梯度结合有机溶剂梯度的反相高效液相色谱(RP-HPLC)进行一维液相色谱分离,按等时间间隔收集馏分并将一个梯度的前段的一个馏分与后段一个馏分混合,然后进行纳升级液相色谱-质谱联用(nanoRPLC-MS/MS)分析.将该方法应用于酵母蛋白质的分离和鉴定,实验结果为:与常规的强阳离子色谱-反相液相色谱-质谱分离鉴定方法相比,采用pH梯度结合有机相梯度的RP-HPLC-RPLC-MS分离鉴定方法多鉴定到567个酵母蛋白质(簇,含有3 035个唯一肽段);其中鉴定到肽段的pI分布范围为3.42～12.01,相对分子质量范围为587.67～3 499.79;蛋白质的pI分布范围为3.82～12.19,相对分子质量范围为3 446.55～432 905.该结果表明这种方法在复杂体系蛋白质组分离鉴定中具有明显的优势,在蛋白质组学研究中有较好的应用前景.","authors":[{"authorName":"马岩","id":"d9df6df7-1e61-4477-a4df-9ee5b0a57a5b","originalAuthorName":"马岩"},{"authorName":"张万军","id":"8411353c-ae4c-402e-9372-98accd21826a","originalAuthorName":"张万军"},{"authorName":"<span style=\"color:red\">卫</span><span style=\"color:red\">军营</span>","id":"9dfcf48d-38cf-44bf-a4c5-e661c3a889c9","originalAuthorName":"卫军营"},{"authorName":"牛明","id":"55d182de-217b-4cad-8071-4930dd10521f","originalAuthorName":"牛明"},{"authorName":"林虹君","id":"2c0b4303-1df1-437c-9d18-8c43106305a1","originalAuthorName":"林虹君"},{"authorName":"秦伟捷","id":"b80109be-b330-46b6-b07d-545cbada878f","originalAuthorName":"秦伟捷"},{"authorName":"张养军","id":"cdf9925f-e47c-40fa-a030-8a4823453ebb","originalAuthorName":"张养军"},{"authorName":"钱小红","id":"eea508d7-4a7c-4621-918e-3f26bf394ffe","originalAuthorName":"钱小红"}],"doi":"10.3724/SP.J.1123.2011.00205","fpage":"205","id":"3f23d383-12bd-4fb6-aa9b-5642a9a13627","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"de4741f2-f6b4-46f9-82de-353fb7eb795e","keyword":"反相高效液相色谱","originalKeyword":"反相高效液相色谱"},{"id":"c573a2e7-a0f2-48b2-a19c-0c7bdee90d7c","keyword":"pH梯度洗脱","originalKeyword":"pH梯度洗脱"},{"id":"05235a18-92ee-4a4f-a50c-c346c7a27721","keyword":"有机溶剂梯度洗脱","originalKeyword":"有机溶剂梯度洗脱"},{"id":"09866dc0-9b5e-475d-88d9-7baef634272e","keyword":"纳升级反相高效液相色谱-串联质谱","originalKeyword":"纳升级反相高效液相色谱-串联质谱"},{"id":"6ea40a8e-96fa-4b47-abf5-22ecd761cee9","keyword":"肽混合物","originalKeyword":"肽混合物"},{"id":"2619c523-048c-48a2-82c8-3fa43675b833","keyword":"酵母","originalKeyword":"酵母"}],"language":"zh","publisherId":"sp201103004","title":"反相高效液相色谱双梯度洗脱分离肽混合物及质谱分析","volume":"29","year":"2011"},{"abstractinfo":"建立了依赖色谱保留时间的智能化选择反应监测质谱方法,并与非依赖色谱保留时间的智能化选择反应监测质谱分析方法对不同体系(牛血清白蛋白酶切物、6种标准蛋白质混合物酶切物、腾冲嗜热菌蛋白提取液酶切物)的分析结果进行了系统比较.结果表明,引入色谱保留时间后的智能化选择反应监测质谱方法能够显著提高肽段及蛋白质的鉴定量,并且在复杂体系(如腾冲嗜热菌蛋白提取液酶切物)中效果尤为明显,鉴定到的肽段及蛋白质的覆盖率可分别达到目标肽段和蛋白质数量的89.62％和92.41％,并且灵敏度高、重复性好,能够实现对质荷比相同但保留时间有差异的肽段的准确鉴定.该方法将在复杂生物样本目标蛋白质组高通量、高灵敏度的鉴定、验证和确认中发挥独特作用.","authors":[{"authorName":"毛心丽","id":"dd8b1968-6cd0-44b0-b5c9-9d093ece3261","originalAuthorName":"毛心丽"},{"authorName":"<span style=\"color:red\">卫</span><span style=\"color:red\">军营</span>","id":"934a7a5d-8138-4e1c-b4ee-d713deeb09bf","originalAuthorName":"卫军营"},{"authorName":"牛明","id":"d8752418-4c4e-44dc-bdd2-dad56cc8a3d7","originalAuthorName":"牛明"},{"authorName":"周廉淇","id":"e88a2363-d09f-49e5-8f19-27c3045e7284","originalAuthorName":"周廉淇"},{"authorName":"王雪颖","id":"28a49eaa-1e22-488e-8bb8-fac6f3daa278","originalAuthorName":"王雪颖"},{"authorName":"佟巍","id":"f69624d0-7de3-41bb-91cd-f3fe85fb79ab","originalAuthorName":"佟巍"},{"authorName":"秦伟捷","id":"6aa308cb-6a0b-4bc0-8d47-6f51b1e6259a","originalAuthorName":"秦伟捷"},{"authorName":"张养军","id":"2098acc3-0063-4a35-a681-bd1168e1a834","originalAuthorName":"张养军"},{"authorName":"钱小红","id":"1e2198df-2a1d-42ce-aa11-9b22cdba1ab9","originalAuthorName":"钱小红"}],"doi":"10.3724/SP.J.1123.2011.11020","fpage":"170","id":"7c735886-578f-4870-9774-609ab8fbbe13","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"9482a656-1186-4d54-9bc8-bb6abd00b571","keyword":"液相色谱","originalKeyword":"液相色谱"},{"id":"b11b4287-843b-45c4-a601-d83825936b81","keyword":"保留时间","originalKeyword":"保留时间"},{"id":"de81f4e6-7e49-43a9-b796-6df1c1565eb5","keyword":"智能化选择反应监测","originalKeyword":"智能化选择反应监测"},{"id":"04f9fbcd-c1d8-4586-84e1-0530dd2b2aa3","keyword":"质谱","originalKeyword":"质谱"},{"id":"e6f1a4da-58ff-488d-8d0d-0baf1854c79b","keyword":"目标蛋白质组学","originalKeyword":"目标蛋白质组学"},{"id":"2ae6e4b0-5730-4587-b517-07365ba94f08","keyword":"腾冲嗜热菌","originalKeyword":"腾冲嗜热菌"}],"language":"zh","publisherId":"sp201202011","title":"依赖色谱保留时间的智能化选择反应监测质谱方法的建立及其初步应用","volume":"30","year":"2012"},{"abstractinfo":"建立了定量肽段串联体蛋白质(concatamers of Q peptides,QconCATs)结合18O同位素标记-多反应监测质谱的蛋白质绝对定量新方法.首先对QconCAT重组蛋白质进行了纯度表征,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)表征结果表明重组蛋白质的纯度在99％以上,相对分子质量约为63.4 kDa.对QconCAT重组蛋白质酶切后的肽段混合物进行质谱分析,并经pFind和pLabel软件处理,验证了目标肽段.还考察了QconCAT重组蛋白质的酶切效率和18O标记效率,并对QconCAT蛋白质结合18O标记-同位素稀释-多反应监测质谱方法进行了评价.实验结果表明,采用该方法对腾冲嗜热厌氧菌(Thermoanaerobacter tengcongensis,TTE)中选定蛋白质的肽段进行绝对含量测定时,相对标准偏差小于20％,准确度较高,说明该方法可用于复杂生物样本中蛋白质的绝对定量.更重要的是所建方法不仅解决了细胞培养氨基酸稳定同位素标记(SILAC)技术的重标试剂价格昂贵的问题,也为定量蛋白质组学提供了一种新的方法.","authors":[{"authorName":"李楠楠","id":"9422f06f-728d-4996-b598-98c8138f2e86","originalAuthorName":"李楠楠"},{"authorName":"周廉淇","id":"8413281e-efee-4fa4-a0c3-bcf65b678663","originalAuthorName":"周廉淇"},{"authorName":"毛心丽","id":"5726f894-b217-4303-a93f-857e109d02c6","originalAuthorName":"毛心丽"},{"authorName":"张姣","id":"a86cb3d8-996c-45da-b079-cfa25edfffd2","originalAuthorName":"张姣"},{"authorName":"<span style=\"color:red\">卫</span><span style=\"color:red\">军营</span>","id":"419a2108-8e3d-4f17-8752-d4b7d039abc8","originalAuthorName":"卫军营"},{"authorName":"林虹君","id":"7faee86b-1420-402b-bf03-0fbf19963fca","originalAuthorName":"林虹君"},{"authorName":"李佳斌","id":"a23bf2e0-8753-4fe4-a349-37dd34ab2e1c","originalAuthorName":"李佳斌"},{"authorName":"田芳","id":"533f8ef8-1346-4fb7-92ef-274d69776fbe","originalAuthorName":"田芳"},{"authorName":"张养军","id":"58321543-ee70-4ab4-9a2a-d9790e5baf42","originalAuthorName":"张养军"}],"doi":"10.3724/SP.J.1123.2013.03057","fpage":"522","id":"4ff71e99-e373-4a96-8deb-60fc25e7be02","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"bcb3bb1b-d0c6-4a59-9039-d8c704d9a21f","keyword":"定量肽段串联体蛋白质","originalKeyword":"定量肽段串联体蛋白质"},{"id":"5d43fb69-7c3f-4c5a-be1d-023b68420c72","keyword":"同位素稀释","originalKeyword":"同位素稀释"},{"id":"cc5717d2-a940-4747-acb1-ff08ca786f2b","keyword":"多反应监测质谱","originalKeyword":"多反应监测质谱"},{"id":"5d3aced2-a29b-41da-84d3-bf2fcf3ffeb8","keyword":"18O标记","originalKeyword":"18O标记"},{"id":"ee4d57d3-e31f-4e81-9a8c-efe03f5c3fce","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"}],"language":"zh","publisherId":"sp201306006","title":"18O同位素标记定量肽段串联体蛋白质结合同位素稀释-多反应监测质谱的蛋白质绝对定量新方法","volume":"31","year":"2013"},{"abstractinfo":"针对攀钢线材厂粗中轧滑动导<span style=\"color:red\">卫</span>不适应优质钢生产的问题,介绍滚动导<span style=\"color:red\">卫</span>的选择和优化设计及其效果.","authors":[{"authorName":"胡尚权","id":"98fb8fd7-376d-4504-a9f0-1f9da4717461","originalAuthorName":"胡尚权"}],"doi":"10.3969/j.issn.1001-1447.2003.04.012","fpage":"39","id":"18529d01-44f2-42b3-a75d-2d4a8d5405b0","issue":"4","journal":{"abbrevTitle":"GTYJ","coverImgSrc":"journal/img/cover/GTYJ.jpg","id":"29","issnPpub":"1001-1447","publisherId":"GTYJ","title":"钢铁研究"},"keywords":[{"id":"f24150c1-49af-40bc-bb38-a879e39ce82d","keyword":"滚动导<span style=\"color:red\">卫</span>","originalKeyword":"滚动导卫"},{"id":"c18ffb4c-12cc-4050-a1f8-691853d8e563","keyword":"轧机","originalKeyword":"轧机"},{"id":"b880c23c-fec8-4151-8aa8-d896b9be0d91","keyword":"优化设计","originalKeyword":"优化设计"}],"language":"zh","publisherId":"gtyj200304012","title":"滚动导<span style=\"color:red\">卫</span>优化设计","volume":"32","year":"2003"},{"abstractinfo":"山东莱芜钢铁集团公司棒材厂在开发无孔型轧制工艺过程中,设计应用了可调组合式滑动进口导<span style=\"color:red\">卫</span>装置,并对滚动进口导<span style=\"color:red\">卫</span>进行了修复改进,保证了无孔型轧制工艺的顺利实施及轧制过程稳定。可调组合式滑动进口导<span style=\"color:red\">卫</span>装置,导<span style=\"color:red\">卫</span>内腔尺寸及安装尺寸可调,适应性、共用性强,具有推广应用价值。","authors":[{"authorName":"黄文初","id":"3ce3421e-1c1c-47b4-98b8-5ce58af97886","originalAuthorName":"黄文初"}],"doi":"","fpage":"44","id":"5cd3d631-6acf-400c-b668-d8d7fa7ca7c5","issue":"7","journal":{"abbrevTitle":"ZGYJ","coverImgSrc":"journal/img/cover/ZGYJ.jpg","id":"87","issnPpub":"1006-9356","publisherId":"ZGYJ","title":"中国冶金"},"keywords":[{"id":"09985ce7-c1da-4f8c-a842-c8395cb4ed9d","keyword":"无孔型轧制","originalKeyword":"无孔型轧制"},{"id":"c6daa204-b310-4afa-9128-5a75cf27de10","keyword":"可调","originalKeyword":"可调"},{"id":"b5861a57-5cf9-40fb-af66-88c3eca02e6b","keyword":"组合式","originalKeyword":"组合式"},{"id":"9d7e4e06-1809-44ba-a906-e89123035716","keyword":"导<span style=\"color:red\">卫</span>设计","originalKeyword":"导卫设计"}],"language":"zh","publisherId":"zgyj201107015","title":"无孔型轧制导<span style=\"color:red\">卫</span>设计","volume":"21","year":"2011"},{"abstractinfo":"在分析棒、线材轧机使用的Gx铸造合金导<span style=\"color:red\">卫</span>辊失效形式的基础上,对不同热处理状态的GJH-2合金和Gx铸造合金,在不同温度下的高温磨损特性进行了对比试验,并将GJH-2合金制成导<span style=\"color:red\">卫</span>辊在轧机上进行了装机试验.试验结果表明,用GJH-2合金代替Gx铸造合金制作导<span style=\"color:red\">卫</span>辊可以大幅度提高导<span style=\"color:red\">卫</span>辊的使用寿命和经济效益,GJH-2合金导<span style=\"color:red\">卫</span>辊的使用寿命是Gx铸造合金辊的16.5倍.","authors":[{"authorName":"陈其伟","id":"3cf35094-99d0-4f3e-a614-49cef90edc1f","originalAuthorName":"陈其伟"}],"doi":"","fpage":"25","id":"4aa9e614-bd31-4949-8d0a-f98755e5c24b","issue":"3","journal":{"abbrevTitle":"GTYJXB","coverImgSrc":"journal/img/cover/GTYJXB.jpg","id":"30","issnPpub":"1001-0963","publisherId":"GTYJXB","title":"钢铁研究学报"},"keywords":[{"id":"31d9b625-f64a-4d48-a554-807461012d09","keyword":"导<span style=\"color:red\">卫</span>辊","originalKeyword":"导卫辊"},{"id":"1957730a-6e1a-494a-a732-46c57e9c9bb2","keyword":"寿命","originalKeyword":"寿命"},{"id":"fe8be149-48f2-49ff-a8ab-1bd188307e9c","keyword":"钢结硬质合金","originalKeyword":"钢结硬质合金"},{"id":"6fe4d901-7bed-4e85-bc8d-93b299d10872","keyword":"高温磨损","originalKeyword":"高温磨损"},{"id":"1a366239-4ba6-4740-abf6-7f30cd66f5b8","keyword":"特性","originalKeyword":"特性"}],"language":"zh","publisherId":"gtyjxb200203006","title":"提高轧机导<span style=\"color:red\">卫</span>辊寿命的研究","volume":"14","year":"2002"},{"abstractinfo":"以Cr、Mo为主要合金元素,用Ni、Mn、Si、V、Ti合金化处理,用Y-K-Na变质处理,研制成功了性能优良的多元高铬钼铸钢,用于制作轧钢机导<span style=\"color:red\">卫</span>板,使用寿命比高镍铬合金导<span style=\"color:red\">卫</span>板提高了50%以上,成本降低30%,综合效益良好.","authors":[{"authorName":"洪桃生","id":"e24fd7bb-8db9-4abd-8e34-ef55031a0016","originalAuthorName":"洪桃生"},{"authorName":"符寒光","id":"e6c8ff31-7457-4ae4-b431-0bdb8dd5a84f","originalAuthorName":"符寒光"}],"doi":"10.3969/j.issn.1001-1447.2000.04.014","fpage":"47","id":"fc408909-b9ff-4bfb-b48a-b9f14114f86b","issue":"4","journal":{"abbrevTitle":"GTYJ","coverImgSrc":"journal/img/cover/GTYJ.jpg","id":"29","issnPpub":"1001-1447","publisherId":"GTYJ","title":"钢铁研究"},"keywords":[{"id":"ba579a28-631c-4529-b1db-812f778c0d8e","keyword":"高铬钼铸钢","originalKeyword":"高铬钼铸钢"},{"id":"b616ed30-7ff4-4d87-9387-8f2c3612f0e3","keyword":"微合金化","originalKeyword":"微合金化"},{"id":"a2793792-b5ca-4636-913c-4de2f64d73e9","keyword":"变质处理","originalKeyword":"变质处理"},{"id":"e4cea0be-1677-432a-968a-b96e028063e3","keyword":"导<span style=\"color:red\">卫</span>板","originalKeyword":"导卫板"}],"language":"zh","publisherId":"gtyj200004014","title":"多元高铬钼铸钢导<span style=\"color:red\">卫</span>板的研制","volume":"","year":"2000"},{"abstractinfo":"介绍了安钢自行设计的高线粗中轧机导<span style=\"color:red\">卫</span>装置的设计原则和特点.","authors":[{"authorName":"解继锋","id":"3c498563-da87-44e9-8ad7-3b7e4fe065f4","originalAuthorName":"解继锋"},{"authorName":"赵自义","id":"b46e8a15-d698-43d6-a7b3-086671c66a7d","originalAuthorName":"赵自义"},{"authorName":"李子林","id":"53a2b4bb-6819-4290-a4df-59fd81463b73","originalAuthorName":"李子林"}],"doi":"10.3969/j.issn.1001-1447.2003.05.008","fpage":"29","id":"4537aec7-5fb6-4eb4-9155-9bea30bb50a6","issue":"5","journal":{"abbrevTitle":"GTYJ","coverImgSrc":"journal/img/cover/GTYJ.jpg","id":"29","issnPpub":"1001-1447","publisherId":"GTYJ","title":"钢铁研究"},"keywords":[{"id":"9c99a858-ef8d-43f6-b144-659f070f07ad","keyword":"高线轧机","originalKeyword":"高线轧机"},{"id":"ce511d53-b0bd-488c-bfcc-ea60dcc5e082","keyword":"导<span style=\"color:red\">卫</span>","originalKeyword":"导卫"},{"id":"8621af20-bcb9-4acb-8fda-c34d2dc6494f","keyword":"设计","originalKeyword":"设计"}],"language":"zh","publisherId":"gtyj200305008","title":"安钢高线粗中轧机导<span style=\"color:red\">卫</span>设计特点","volume":"31","year":"2003"},{"abstractinfo":"回顾了导<span style=\"color:red\">卫</span>装置的发展历程,全面介绍了导辊的材料使用状况,分析失效形式并找出失效原因,阐述了导辊的选材和材料研究方向.","authors":[{"authorName":"向勇","id":"d242f7a0-6d64-48f7-89b5-bf8e83a30254","originalAuthorName":"向勇"},{"authorName":"任立军","id":"8d789b39-eb84-4870-9e8e-3737d93980f0","originalAuthorName":"任立军"}],"doi":"10.3969/j.issn.1001-1447.1999.04.011","fpage":"43","id":"99174195-bf09-4e9b-b959-9bea978f3234","issue":"4","journal":{"abbrevTitle":"GTYJ","coverImgSrc":"journal/img/cover/GTYJ.jpg","id":"29","issnPpub":"1001-1447","publisherId":"GTYJ","title":"钢铁研究"},"keywords":[{"id":"52ccb594-358f-433a-a907-e24bd233906e","keyword":"导<span style=\"color:red\">卫</span>","originalKeyword":"导卫"},{"id":"685a19c0-bf74-4a77-bd4e-2fc7dd467214","keyword":"导辊","originalKeyword":"导辊"},{"id":"cc5d5c31-b799-43d6-96a5-236560740ff5","keyword":"抗磨材料","originalKeyword":"抗磨材料"}],"language":"zh","publisherId":"gtyj199904011","title":"导<span style=\"color:red\">卫</span>装置的发展及导辊材料的研究","volume":"","year":"1999"},{"abstractinfo":"应用合金粉末等离子喷焊技术进行工艺试验,获得了Fe50、Ni60及NiWC25三种优质合金强化喷焊层.热轧导<span style=\"color:red\">卫</span>板表面等离子喷焊NiWC25工业试验表明:具有NiWC25等离子喷焊强化层的Q235导<span style=\"color:red\">卫</span>板使用寿命高,平均过钢量达10万吨,约为灰铸铁HT200导<span style=\"color:red\">卫</span>板的5倍.","authors":[{"authorName":"王银军","id":"796f13e3-0053-44f2-8f0e-ad46add9593f","originalAuthorName":"王银军"}],"doi":"10.3969/j.issn.1001-3660.2005.04.021","fpage":"58","id":"ea57e55d-7fa0-4c90-81c6-73292a813d03","issue":"4","journal":{"abbrevTitle":"BMJS","coverImgSrc":"journal/img/cover/BMJS.jpg","id":"3","issnPpub":"1001-3660","publisherId":"BMJS","title":"表面技术   "},"keywords":[{"id":"ba66aeb5-001c-458f-bd31-3bd2af044c00","keyword":"等离子喷焊","originalKeyword":"等离子喷焊"},{"id":"7de49f8e-a453-46e7-b7ce-2e894f117aec","keyword":"合金粉末","originalKeyword":"合金粉末"},{"id":"70ac2410-94fa-4d4a-a4ab-01178a4243e6","keyword":"导<span style=\"color:red\">卫</span>板","originalKeyword":"导卫板"}],"language":"zh","publisherId":"bmjs200504021","title":"等离子喷焊工艺试验及其在热轧导<span style=\"color:red\">卫</span>板上的应用","volume":"34","year":"2005"}],"totalpage":4,"totalrecord":35}