{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"蛋白质的糖基化是最重要的翻译后修饰之一,与蛋白质结构和功能的关系密切.凝集素亲和色谱是蛋白质糖基化研究中很常用的工具,不同的凝集素可以对不同的单糖或寡糖有特异的富集作用.麦胚凝集素(WGA)由于其特异作用的糖型广泛存在而成为使用最多的凝集素之一.在本研究中,发现将WGA用于糖肽亲和富集会导致部分肽段的降解,从而导致后续的肽段序列分析的失败.本文用4种标准蛋白质对这种现象进行了验证,结果表明肽段的降解可以发生在多个位点,其中较多地发生在酪氨酸、苯丙氨酸及亮氨酸的羧基端.这一结果提示:在糖蛋白质组研究中,如果应用WGA富集糖肽并采用质谱进行鉴定,则采用半酶切或非特异性酶切的检索策略更为合适.","authors":[{"authorName":"卢庄","id":"06b9a22b-a66d-473e-b551-c4c02ee89d15","originalAuthorName":"卢庄"},{"authorName":"王京兰","id":"6c58b6bd-f7c8-4cf4-a7ce-617ebd8f1988","originalAuthorName":"王京兰"},{"authorName":"贾伟","id":"5e271b2c-fd73-4ad5-ac08-35a2abf91551","originalAuthorName":"贾伟"},{"authorName":"杨兵","id":"ffc1f5e7-1bc1-4efd-8844-23366660650e","originalAuthorName":"杨兵"},{"authorName":"蔡耘","id":"124a0f2c-2894-4664-a998-bb22aa7f92ae","originalAuthorName":"蔡耘"},{"authorName":"邓玉林","id":"4f51ef9f-004e-4613-82c3-331d05d8194e","originalAuthorName":"邓玉林"},{"authorName":"张玉奎","id":"4e80c736-881a-487d-879e-ac77abd88d2f","originalAuthorName":"张玉奎"},{"authorName":"钱小红","id":"2c9ab802-b9d5-4a8e-83f3-e79313370c7f","originalAuthorName":"钱小红"}],"doi":"10.3321/j.issn:1000-8713.2009.01.003","fpage":"19","id":"f6e5e65b-4ec2-4da5-917e-88a46d30be61","issue":"1","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"0f640856-f593-41f2-8db5-0b9991eaa3ce","keyword":"麦胚凝集素亲和色谱","originalKeyword":"麦胚凝集素亲和色谱"},{"id":"5622e8ab-ee5c-4405-9bcb-3aa3f34599f4","keyword":"糖肽","originalKeyword":"糖肽"},{"id":"80a40d46-3613-472c-a619-7fb935edfbe4","keyword":"肽键","originalKeyword":"肽键"},{"id":"a35ac0c2-0ddf-49d8-85c0-ebf4b1ce8d09","keyword":"断裂","originalKeyword":"断裂"},{"id":"18d6e2a4-ff47-47da-9b5b-0f85aed4b979","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"}],"language":"zh","publisherId":"sp200901003","title":"麦胚凝集素亲和色谱富集糖肽过程中的肽键裂解","volume":"27","year":"2009"},{"abstractinfo":"二氧化钛富集法作为目前使用最为广泛的金属氧化物富集磷酸肽的方法,在富集过程中常常对富含天冬氨酸和谷氨酸的酸性非磷酸化肽段存在一定的非特异性吸附作用.这些肽段与磷酸化肽段一同被富集,降低了磷酸肽富集的选择性.传统方法中使用的非特异性吸附抑制剂常会对质谱的电喷雾离子源造成污染,因而限制了其在液相色谱-质谱联用(LC-MS)系统中的应用.本研究将天冬氨酸作为一种新型的非特异性吸附抑制剂加入到二氧化钛富集体系中,并分别对3种和9种标准蛋白质酶切肽段混合物进行富集实验,同时与添加另一种非特异性吸附抑制剂--谷氨酸以及不添加任何非特异性吸附抑制剂的富集体系进行了富集效果的比较.结果表明,天冬氨酸可以有效地提高二氧化钛对磷酸肽富集的选择性.将添加天冬氨酸的二氧化钛富集体系应用于鼠肝全蛋白质磷酸肽的富集中,同样取得了很好的效果,表明天冬氨酸在复杂的生物样本的磷酸肽富集中也同样具有良好的应用前景.此外,由于天冬氨酸在反相色谱中极易被洗脱去除,从而避免了传统抑制剂对LC-MS系统离子源的污染问题.","authors":[{"authorName":"迟明","id":"5db75c52-4cc1-477e-8354-aa6c879a0fc2","originalAuthorName":"迟明"},{"authorName":"毕炜","id":"6463f68e-1a1d-4d15-bae2-ca977165cb57","originalAuthorName":"毕炜"},{"authorName":"卢庄","id":"bb1cde5e-538b-48a7-92d4-fa0fe2285c43","originalAuthorName":"卢庄"},{"authorName":"宋丽娜","id":"43b858bd-c2cb-4425-b598-5a94343bab51","originalAuthorName":"宋丽娜"},{"authorName":"贾伟","id":"207f9db0-16d0-40fa-957b-1ca4221c25c2","originalAuthorName":"贾伟"},{"authorName":"张养军","id":"7921def7-2014-4bf8-9155-01b156a91e2d","originalAuthorName":"张养军"},{"authorName":"钱小红","id":"e5afc912-577b-4b14-903e-ab1d55acc606","originalAuthorName":"钱小红"},{"authorName":"蔡耘","id":"492c2de0-be19-4dbe-9854-8933805848bf","originalAuthorName":"蔡耘"}],"doi":"","fpage":"152","id":"15faf714-c05f-4e3b-9f3c-60470da974b7","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"763275dd-4595-48db-b5ea-3bdd54e74f32","keyword":"二氧化钛","originalKeyword":"二氧化钛"},{"id":"1eef5434-aae1-4411-b72c-c5cb96e3a6c6","keyword":"富集","originalKeyword":"富集"},{"id":"8c93ab5b-ff00-4a92-acb0-7c1bdd868768","keyword":"非特异性吸附抑制剂","originalKeyword":"非特异性吸附抑制剂"},{"id":"bcb13d9b-52f6-426a-af1a-4a5a82b81ce5","keyword":"天冬氨酸","originalKeyword":"天冬氨酸"},{"id":"b3162b21-5178-4556-9847-99c9af25c88e","keyword":"磷酸肽","originalKeyword":"磷酸肽"},{"id":"fa7ecbcf-94e2-4a0c-b232-1704f91f82e9","keyword":"鼠肝","originalKeyword":"鼠肝"}],"language":"zh","publisherId":"sp201002009","title":"天冬氨酸作为非特异性吸附抑制剂在二氧化钛选择性富集磷酸肽中的应用","volume":"28","year":"2010"},{"abstractinfo":"为了在短时间内获得相对含量高的磷酸化肽段,以标准磷酸化蛋白质为模型对强阳离子交换色谱(SCX)分离磷酸化肽段体系的缓冲溶液和梯度设置进行了研究,并用酵母酶切肽段混合物考察了该路线在较复杂的样品中的应用.实验结果表明优化后的体系能够在30 min内分离出磷酸肽段,而且非磷酸化肽段的干扰很少,这样便相对提高了磷酸化肽段在质谱仪中的响应强度,重要的是该体系可以对复杂样品进行很好的分离.这说明SCX用于规模化磷酸化肽段富集的策略是可行的.本研究为磷酸化蛋白质组学规模化分析提供了实用技术.","authors":[{"authorName":"隋少卉","id":"a7a7ffda-b0b0-4cc0-800c-5adb6215c46f","originalAuthorName":"隋少卉"},{"authorName":"王京兰","id":"447b3303-9c36-459f-aa9c-06e4e3f96925","originalAuthorName":"王京兰"},{"authorName":"卢庄","id":"e9737aae-8c90-4f20-a3c6-586caa063500","originalAuthorName":"卢庄"},{"authorName":"蔡耘","id":"9b9c9a32-03ba-4639-bd46-57aea4f7f0fe","originalAuthorName":"蔡耘"},{"authorName":"张养军","id":"15f37bb6-6a65-44d2-ae04-af199183b2eb","originalAuthorName":"张养军"},{"authorName":"蔚文峰","id":"b240c187-fc41-4224-aa11-b39ef46591e5","originalAuthorName":"蔚文峰"},{"authorName":"钱小红","id":"17b9a151-7253-4270-8785-69d980bf866e","originalAuthorName":"钱小红"}],"doi":"10.3321/j.issn:1000-8713.2008.02.012","fpage":"195","id":"f0a96905-9339-445d-8c19-bc67c2605657","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"9639324f-90d3-4269-b115-24ebbe5fa34b","keyword":"磷酸化","originalKeyword":"磷酸化"},{"id":"a49a9f5a-a94b-4148-bcf3-31e79fc77a85","keyword":"位点","originalKeyword":"位点"},{"id":"cf070b23-026b-4e47-b864-f423428b448d","keyword":"强阳离子交换色谱","originalKeyword":"强阳离子交换色谱"},{"id":"68bae8b0-8f3d-4a0f-91c4-713024f08434","keyword":"富集","originalKeyword":"富集"},{"id":"8bf355a0-4c10-4062-8689-5734092fceeb","keyword":"蛋白质组学","originalKeyword":"蛋白质组学"}],"language":"zh","publisherId":"sp200802012","title":"基于强阳离子交换色谱分离的磷酸化肽段富集策略","volume":"26","year":"2008"},{"abstractinfo":"岩体结构特征研究是采场稳定性分析及评价的基础.根据蚕庄金矿上庄矿区岩体结构面调查分析结果,对矿区岩体潜在滑塌块体的识别和在矿块回采中的应用作了论述,能为矿山安全采矿提供一定的依据.","authors":[{"authorName":"杨锡祥","id":"cefcf979-933a-4aab-b549-9a3bda4c97ca","originalAuthorName":"杨锡祥"},{"authorName":"周英芳","id":"a8ff9ec3-a016-4cda-8658-4443681a8f4c","originalAuthorName":"周英芳"},{"authorName":"赵小稚","id":"6202d41b-e4dc-48e0-82c0-2cb0f5fa2a13","originalAuthorName":"赵小稚"}],"doi":"10.3969/j.issn.1001-1277.2010.02.007","fpage":"27","id":"a07bde77-3d25-4c47-bb83-63497ace0ec9","issue":"2","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"40b38bae-5868-48da-b5b2-10d63ea102e3","keyword":"岩体","originalKeyword":"岩体"},{"id":"e898b7c3-d21a-47a9-b621-db8b5600f0ef","keyword":"结构面","originalKeyword":"结构面"},{"id":"5ab3331a-6580-450f-9ae8-9127821c69c7","keyword":"滑塌块体","originalKeyword":"滑塌块体"},{"id":"c80763d4-3e6a-47cd-b7b3-e21ac95d27bd","keyword":"安全回采","originalKeyword":"安全回采"}],"language":"zh","publisherId":"huangj201002007","title":"蚕庄金矿上庄矿区岩体结构面分析及应用","volume":"31","year":"2010"},{"abstractinfo":"通过对归来庄金矿床探采对比的研究,确定了较合理的生产勘探方法,对今后矿山地质探矿及深部开采具有重要指导意义.","authors":[{"authorName":"刘林","id":"636b2429-e8f1-4972-9344-1a9d574a1feb","originalAuthorName":"刘林"},{"authorName":"陈为友","id":"d50a0b6c-0a4b-4057-91d0-963cbdc37c8c","originalAuthorName":"陈为友"},{"authorName":"尹士增","id":"bfb4a0c6-d44e-4202-a31f-4c82570b60c1","originalAuthorName":"尹士增"},{"authorName":"廉永","id":"562a43a1-5188-48b8-8c61-81921664dba1","originalAuthorName":"廉永"}],"doi":"10.3969/j.issn.1001-1277.2001.05.003","fpage":"11","id":"878d3fd7-b170-448a-be3c-9631c9cfdd47","issue":"5","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"06347b0e-2e57-421c-aaa5-a94e12471c32","keyword":"探采对比","originalKeyword":"探采对比"},{"id":"12146576-59d1-4e7b-9f19-34982a2cdc73","keyword":"初步研究","originalKeyword":"初步研究"},{"id":"73010682-be90-434e-ac00-46413cf66a79","keyword":"归来庄金矿","originalKeyword":"归来庄金矿"}],"language":"zh","publisherId":"huangj200105003","title":"平邑归来庄金矿床探采对比初步研究","volume":"22","year":"2001"},{"abstractinfo":"运用地质观测和统计分析研究,讨论了山东大尹格庄金矿床NWW向大尹格庄断裂的控矿作用.研究表明,大尹格庄断裂对矿化类型、强度和矿体产状都有明显的控制作用.NWW向大尹格庄断裂以北的Ⅱ号矿体群以金矿化为主,断裂南部Ⅰ号矿体群以银矿化叠加早期金矿化为特征.此外,研究还发现,大尹格庄断裂本身也存在金矿化,这对深化对该区矿床成因的认识和指导找矿预测都有一定的意义.","authors":[{"authorName":"潘红伟","id":"cfd2ffbc-3c14-4ba8-acee-2236512398f8","originalAuthorName":"潘红伟"},{"authorName":"张瑞忠","id":"499f1785-9507-47cc-b37e-a22d1be4445b","originalAuthorName":"张瑞忠"},{"authorName":"樊明玉","id":"357781ea-702c-41a6-a887-72016ead099e","originalAuthorName":"樊明玉"}],"doi":"10.3969/j.issn.1001-1277.2008.01.005","fpage":"21","id":"125cfddb-de49-43e9-949d-5160bbe74e26","issue":"1","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"3e701120-25e3-4287-897f-3c7f1fa6a6e3","keyword":"大尹格庄断裂","originalKeyword":"大尹格庄断裂"},{"id":"48402ab9-dfd8-4b5d-8b94-f7f2850a9afb","keyword":"NWW向断裂","originalKeyword":"NWW向断裂"},{"id":"2930d870-0187-4afe-ad61-3e2bff71c8eb","keyword":"控矿构造","originalKeyword":"控矿构造"},{"id":"bf1c20db-0a5d-4759-8b6c-93c00877b4fd","keyword":"大尹格庄金矿","originalKeyword":"大尹格庄金矿"}],"language":"zh","publisherId":"huangj200801005","title":"山东大尹格庄金矿NWW向断裂控矿作用浅析","volume":"29","year":"2008"},{"abstractinfo":"防治水是矿山建设施工的重要环节.南李庄矿属于水文地质条件复杂的大水岩溶矿山,介绍了主井掘进-129 m水平突水及治水过程,在查清该矿区水文地质条件及矿井突水要因的基础上,为综合防治竖井掘进期间通过特殊地质构造段突水,提出相应的主井掘进帷幕注浆防治水技术及措施,并在掘进工程中收到了良好的应用效果,对类似特殊条件下的矿山井巷掘进工程具有借鉴意义.","authors":[{"authorName":"董华兴","id":"c08faef8-222f-4269-ab57-068e213c04a0","originalAuthorName":"董华兴"},{"authorName":"郑翠敏","id":"71818941-893c-450e-8900-6d9f7dc6d4f4","originalAuthorName":"郑翠敏"},{"authorName":"董凤霞","id":"ce559c6e-5477-4acc-96d6-55cc93294757","originalAuthorName":"董凤霞"},{"authorName":"韩建国","id":"d72c3f8a-9086-4000-8209-5e6a1f371d1d","originalAuthorName":"韩建国"}],"doi":"10.11792/hj20130209","fpage":"35","id":"50da3555-9182-4c83-b8b8-7c218b723d08","issue":"2","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"c8ee1711-e9ee-4c00-a471-1354d308bfe1","keyword":"大水矿山","originalKeyword":"大水矿山"},{"id":"5da58d6c-dff5-4998-b645-b63ba8ec37c0","keyword":"主井掘进","originalKeyword":"主井掘进"},{"id":"a29b56b2-06f6-49a2-9412-0a6cbd37c8f5","keyword":"特殊地质构造","originalKeyword":"特殊地质构造"},{"id":"49c4c65b-3993-4060-b81d-5caf117fa377","keyword":"突水","originalKeyword":"突水"},{"id":"5f22c680-f6b2-4312-8b46-fe823c71eddb","keyword":"防治","originalKeyword":"防治"}],"language":"zh","publisherId":"huangj201302009","title":"南李庄矿主井施工突水防治技术","volume":"34","year":"2013"},{"abstractinfo":"文中用数值模拟的方法分析了矿房倾角对蚕庄金矿倾斜试验采场稳定性的影响,找到了最佳的矿房倾角,得出了有价值的结论.","authors":[{"authorName":"安然","id":"eb59fc27-4023-4c9d-801c-ffbec5a44bbb","originalAuthorName":"安然"},{"authorName":"王保学","id":"f2767b10-4cd8-4b56-aaa9-aabd894534f5","originalAuthorName":"王保学"},{"authorName":"扬同","id":"b76378c0-3100-4303-bdbb-8b597dbc2d0d","originalAuthorName":"扬同"}],"doi":"10.3969/j.issn.1001-1277.2001.02.005","fpage":"16","id":"c4133e77-684a-4213-b763-399af8c1e760","issue":"2","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"dd846364-ba70-45c6-8b88-b010dc9d8cac","keyword":"倾斜矿房","originalKeyword":"倾斜矿房"},{"id":"f5f14367-2d0d-4054-9648-25eaa5f498e2","keyword":"数值模拟","originalKeyword":"数值模拟"},{"id":"e5b0c150-f742-485b-8af3-7d5c7007868f","keyword":"稳定性分析","originalKeyword":"稳定性分析"}],"language":"zh","publisherId":"huangj200102005","title":"蚕庄金矿倾斜矿房倾角对其稳定性的影响","volume":"22","year":"2001"},{"abstractinfo":"针对蚕庄金矿上庄矿区深部缓倾斜中厚Ⅱ号矿体的赋存及开采技术条件,详细介绍了竖分条采矿法矿块布置及参数、回采及充填工艺,试验应用取得了较上向水平分层尾砂充填采矿法更好的回采效果.值得类似条件矿山借鉴.","authors":[{"authorName":"王兴涛","id":"036e21c0-259e-4e45-a3eb-686158c77e1f","originalAuthorName":"王兴涛"},{"authorName":"郭建军","id":"b1454f12-6dfb-4c1a-9e74-e1fb9a552a9e","originalAuthorName":"郭建军"},{"authorName":"杨玉泉","id":"22b5dc93-d30f-4f1c-b769-f5ecd4e8eae9","originalAuthorName":"杨玉泉"},{"authorName":"浦玉传","id":"7ebbbf24-5cf6-43b1-99a3-9707447e843a","originalAuthorName":"浦玉传"},{"authorName":"李昊","id":"3dd8e1ba-94be-4a20-a3a0-a56418fde666","originalAuthorName":"李昊"}],"doi":"10.3969/j.issn.1001-1277.2008.09.008","fpage":"26","id":"dac718fe-d78e-4a3e-a88b-dafc1be118bd","issue":"9","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"2302b4cd-1c80-4c36-ab83-44cff96a290c","keyword":"竖分条","originalKeyword":"竖分条"},{"id":"f84ac425-f394-4f5a-9edb-7ef107e9b5c2","keyword":"缓倾斜","originalKeyword":"缓倾斜"},{"id":"816f1eb8-8a40-4a67-8e36-fbb9e22f657c","keyword":"中厚矿体","originalKeyword":"中厚矿体"},{"id":"c0f837cb-9812-4a37-ad9b-4d53670fa614","keyword":"蚕庄金矿","originalKeyword":"蚕庄金矿"}],"language":"zh","publisherId":"huangj200809008","title":"竖分条回采工艺在蚕庄金矿缓倾斜中厚矿体的应用实践","volume":"29","year":"2008"},{"abstractinfo":"大尹格庄金矿床为招平断裂带中部的大型蚀变岩型金矿床,矿体的产出严格受招平断裂带控制.文中利用显微构造特征、显微运动学标志观测以及显微裂隙统计计算,来探讨大尹格庄金矿床控矿构造的变形、演化及与金成矿的关系.研究表明:大尹格庄金矿床控矿构造以脆-韧性变形为特征;显微运动学标志揭示控矿断裂发生过左行剪切向右行剪切的转变;显微裂隙统计计算表明,成矿前后主应力方位发生NWW→NE→NW的转变,伴随着构造差应力大→小→大的变化.金成矿作用发生在剪压变形向剪张变形转换阶段.","authors":[{"authorName":"高帮飞","id":"756c2a73-88a1-4bde-8e88-951df2e27179","originalAuthorName":"高帮飞"},{"authorName":"杨立强","id":"a10e9a93-359b-4cbe-b020-315d6be980fa","originalAuthorName":"杨立强"},{"authorName":"王庆飞","id":"39a67aa2-76c8-4bc2-87c3-bdb0269ed006","originalAuthorName":"王庆飞"}],"doi":"10.3969/j.issn.1001-1277.2007.01.003","fpage":"9","id":"ace2e0ac-4344-463c-a407-da34395212ad","issue":"1","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"e8f20066-1227-4a08-97da-b1ae3d56b0a5","keyword":"显微构造","originalKeyword":"显微构造"},{"id":"bcd8d624-28be-446b-b917-4d6b205dbdc8","keyword":"脆-韧性变形","originalKeyword":"脆-韧性变形"},{"id":"e4286d72-24bd-4a9a-8ac4-4cbf77f1f14e","keyword":"应力场转换","originalKeyword":"应力场转换"},{"id":"54f46cfc-6760-4944-bd6a-5fb7896f9dff","keyword":"大尹格庄金矿床","originalKeyword":"大尹格庄金矿床"},{"id":"8554f56b-3647-4db9-8c0a-6473e8e9490b","keyword":"胶东","originalKeyword":"胶东"}],"language":"zh","publisherId":"huangj200701003","title":"胶东大尹格庄金矿床控矿显微构造特征","volume":"28","year":"2007"}],"totalpage":8,"totalrecord":76}