{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"用HPLC/DAD/MS2联用仪,鉴定了板蓝根注射液中含有的核苷类物质.色谱条件:Luna C18色谱柱(250 mm×4.6 mm i.d., 5 μm);甲醇-水梯度洗脱,流量0.8 mL/min; 柱温25 ℃.用二极管阵列检测器记录各个色谱峰的紫外吸收光谱,色谱检测波长254 nm.质谱条件:电喷雾离子源(ESI);正离子检测;扫描范围m/z 50～800.记录质量色谱图和各个色谱峰的一级、二级质谱图,并对质谱结果进行解析,通过与对照品比较,确定板蓝根注射液样品中含有腺苷、鸟<span style=\"color:red\">苷</span>、<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>、胞<span style=\"color:red\">苷</span>及腺嘌呤等5种成分.","authors":[{"authorName":"金郁","id":"15e95cc3-9f05-4093-8d60-2e56c1422d0f","originalAuthorName":"金郁"},{"authorName":"肖珊珊","id":"9607ac53-896c-43c1-bcfc-54a24493eb88","originalAuthorName":"肖珊珊"},{"authorName":"孙毓庆","id":"143b4c2a-acff-41b7-a83b-d68738766112","originalAuthorName":"孙毓庆"}],"doi":"10.3321/j.issn:1000-8713.2003.06.006","fpage":"558","id":"dd8218e2-df9a-4949-80f7-b9f918b8eb2a","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"c3f542cf-7fd6-4a9e-aae8-5e4c15e09c9b","keyword":"液相色谱","originalKeyword":"液相色谱"},{"id":"5de50205-a921-4e9a-a1db-8fae28ccdaa0","keyword":"质谱","originalKeyword":"质谱"},{"id":"82cd8a76-1ca1-4f7a-983b-594dc84d379e","keyword":"腺苷","originalKeyword":"腺苷"},{"id":"1bd1bae7-2a7f-4095-8d0b-74c68e8c444a","keyword":"鸟<span style=\"color:red\">苷</span>","originalKeyword":"鸟苷"},{"id":"541c4cdc-dcc7-4606-9e78-f9774f87b3f2","keyword":"<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>","originalKeyword":"尿苷"},{"id":"3082146c-d8aa-4000-9b68-cc4f14fb2cf6","keyword":"胞<span style=\"color:red\">苷</span>","originalKeyword":"胞苷"},{"id":"de388f41-7638-48a2-8734-08bfc1eafd55","keyword":"腺嘌呤","originalKeyword":"腺嘌呤"},{"id":"7f57da7f-4562-4c63-8b81-c7c6043d6bcb","keyword":"板蓝根注射液","originalKeyword":"板蓝根注射液"},{"id":"144430df-4f0a-44a8-8e7f-6f395850acc8","keyword":"中药","originalKeyword":"中药"}],"language":"zh","publisherId":"sp200306006","title":"高效液相色谱/二极管阵列检测/质谱/质谱(HPLC/DAD/MS2)联用在板蓝根注射液成分鉴定中的应用","volume":"21","year":"2003"},{"abstractinfo":"考察了10 ml丙酮-四氢呋喃(85/15,体积比)混合溶剂体系中,各关键因素对脂肪酶Lipozyme TL IM催化5-氟<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>5’-棕榈酸酯合成反应的影响.结果表明,添加分子筛能有效除去溶剂中的水分,从而极人地提高了目标产物收率.在最优反应条件下,该体系被成功放大至200 ml (5-氟<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>克级用量),产物收率高达99％；同时脂肪酶Lipozyme TL IM表现出优异的操作稳定性,重复使用10批次后,产物收率仍可达97％.另外,填充床反应器的体积生产效率为22.4 g/(L·h),远高于批次反应时的0.5 g/(L·h).","authors":[{"authorName":"侯雪丹","id":"60bc8744-f556-409f-9b40-314921744938","originalAuthorName":"侯雪丹"},{"authorName":"张毅","id":"5403d37a-7b2b-402a-b2ea-f5dd9a9f1523","originalAuthorName":"张毅"},{"authorName":"刘欢","id":"c6855b95-a3dc-4929-b770-8cdecb0db157","originalAuthorName":"刘欢"},{"authorName":"李宁","id":"6c88a4a9-6008-45ce-b776-b410ad0630a6","originalAuthorName":"李宁"},{"authorName":"宗敏华","id":"19e4cadc-4a9b-462a-8b5d-ade2adb131ab","originalAuthorName":"宗敏华"}],"doi":"10.3724/SP.J.1088.2011.10739","fpage":"1733","id":"6dd69962-d49e-40a7-b246-72dc9c7eb6eb","issue":"11","journal":{"abbrevTitle":"CHXB","coverImgSrc":"journal/img/cover/CHXB.jpg","id":"18","issnPpub":"0253-9837","publisherId":"CHXB","title":"催化学报   "},"keywords":[{"id":"4b742b05-a82b-4785-93f7-d0d6a307328e","keyword":"5-氟<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>","originalKeyword":"5-氟尿苷"},{"id":"04734365-afdc-4014-9b45-ca50bfe71b62","keyword":"5’-棕榈酸酯","originalKeyword":"5’-棕榈酸酯"},{"id":"5cb6ce63-08c2-48a2-b883-e779b9726f80","keyword":"固定化脂肪酶","originalKeyword":"固定化脂肪酶"},{"id":"30738d79-59fa-448d-92d8-c6ca8a2ba3b9","keyword":"区域选择性","originalKeyword":"区域选择性"},{"id":"38798767-c672-4802-92d8-30090de38004","keyword":"棕榈酰化","originalKeyword":"棕榈酰化"},{"id":"7956bb72-476f-4bdc-8dae-04d7a981a1ea","keyword":"填充床反应器","originalKeyword":"填充床反应器"}],"language":"zh","publisherId":"cuihuaxb201111010","title":"5-氟<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>5’-棕榈酸酯的酶法合成","volume":"32","year":"2011"},{"abstractinfo":"建立了利用高效液相色谱分离分析谷氨酸棒杆菌细胞内<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>二磷酸(UDP)葡萄糖与UDP半乳糖的新方法,考察了不同色谱柱、不同分离模式及不同流动相组成对UDP葡萄糖和UDP半乳糖的保留特性及分离度的影响.结果表明,在Zorbax NH2柱(250 mm×4.6 mm i.d.,5 μm)上以0.125 mol/L KH2PO4缓冲液(pH 3.6)-乙腈(体积比为40∶60)溶液为流动相,可使谷氨酸棒杆菌细胞内的两种中间代谢产物UDP葡萄糖与UDP半乳糖在22 min内达到基线分离.该方法对UDP葡萄糖和UDP半乳糖的检测限分别为3.2和3.5 ng.UDP葡萄糖和UDP半乳糖样品的平均回收率分别为98.26%和99.02%,相对标准偏差分别为2.71%和3.54%.","authors":[{"authorName":"戴军","id":"97182627-8f75-4d14-b762-6b7910e62421","originalAuthorName":"戴军"},{"authorName":"关惠琴","id":"03cc8b09-f33d-4a9f-9a2b-4e9d447769ee","originalAuthorName":"关惠琴"},{"authorName":"陈尚卫","id":"62fa27d5-08bf-4562-9eb1-acc3ef7828f0","originalAuthorName":"陈尚卫"},{"authorName":"堵国成","id":"97a9256e-3a0b-4746-ad27-d3b43b414591","originalAuthorName":"堵国成"},{"authorName":"陈坚","id":"8683dc94-a526-4218-a786-dbf147c0c520","originalAuthorName":"陈坚"}],"doi":"10.3321/j.issn:1000-8713.2003.06.011","fpage":"580","id":"1e397d21-62a4-4429-9307-57afce1092fc","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"c8766239-5df3-4bb6-b82f-1d02e64a99ff","keyword":"高效液相色谱法","originalKeyword":"高效液相色谱法"},{"id":"caa0e396-2579-480f-8ba2-a7b97f28813e","keyword":"<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>二磷酸葡萄糖","originalKeyword":"尿苷二磷酸葡萄糖"},{"id":"be0f6106-b458-4130-8f3c-d0fe4c8a3f35","keyword":"<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>二磷酸半乳糖","originalKeyword":"尿苷二磷酸半乳糖"},{"id":"af046cd8-7f09-46e1-b8c8-b80ae97ad252","keyword":"谷氨酸棒杆菌","originalKeyword":"谷氨酸棒杆菌"}],"language":"zh","publisherId":"sp200306011","title":"高效液相色谱法分析谷氨酸棒杆菌细胞内<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>二磷酸葡萄糖和<span style=\"color:red\">尿</span><span style=\"color:red\">苷</span>二磷酸半乳糖","volume":"21","year":"2003"},{"abstractinfo":"研究了混合溶剂体系中有机溶剂种类和含量对牛肝β-半乳糖苷酶催化5-氟-2'-脱氧<span style=\"color:red\">尿苷</span>区域选择性半乳糖基化反应的影响.结果表明,在含10%(体积分数)有机溶剂体系中,该酶稳定性差,失活严重.减少有机溶剂含量可显著减轻其对该酶的毒害作用.然而,有机溶剂的添加对酶促糖基化反应的区域选择性的影响很小(均保持在99%以上).牛肝β-半乳糖苷酶在混合溶剂体系中的催化性能与有机溶剂的介电常数有良好的相关性,其中在含二甲亚砜(DMSO)体系中该酶催化活性最高.当体系中DMSO含量≤5%时,目标产物收率达71%.","authors":[{"authorName":"叶敏","id":"df6d5ec9-7695-47f9-9d80-37fc0b534ae8","originalAuthorName":"叶敏"},{"authorName":"刘秋萍","id":"345cd5d1-708f-4f2a-a184-24da595a7fe4","originalAuthorName":"刘秋萍"},{"authorName":"李宁","id":"d5380755-d13b-40af-b028-bc2e04ddea78","originalAuthorName":"李宁"},{"authorName":"宗敏华","id":"a85b97d7-6508-4104-83ee-25576d0d03e7","originalAuthorName":"宗敏华"}],"doi":"10.3724/SP.J.1088.2011.01150","fpage":"1063","id":"81846ff1-56c9-4045-ba72-793e86bf4b44","issue":"6","journal":{"abbrevTitle":"CHXB","coverImgSrc":"journal/img/cover/CHXB.jpg","id":"18","issnPpub":"0253-9837","publisherId":"CHXB","title":"催化学报   "},"keywords":[{"id":"a7f5ea6c-c884-4af0-b7ac-1d990a1f41f7","keyword":"β-半乳糖苷酶","originalKeyword":"β-半乳糖苷酶"},{"id":"1e4d4163-7578-43e5-bd41-0a03e7c1140a","keyword":"糖基化反应","originalKeyword":"糖基化反应"},{"id":"bc604370-135b-4172-9758-0a9dfbeae345","keyword":"区域选择性","originalKeyword":"区域选择性"},{"id":"dc5b7364-bea0-4141-811d-d82ac5d630c2","keyword":"5-氟-2'-脱氧<span style=\"color:red\">尿苷</span>","originalKeyword":"5-氟-2'-脱氧尿苷"},{"id":"4ac3330f-e231-4cd6-a100-87ffdf67f79f","keyword":"有机溶剂","originalKeyword":"有机溶剂"}],"language":"zh","publisherId":"cuihuaxb201106026","title":"混合溶剂体系中牛肝β-半乳糖苷酶催化5-氟-2'-脱氧<span style=\"color:red\">尿苷</span>区域选择性半乳糖基化反应","volume":"32","year":"2011"},{"abstractinfo":"报道了<span style=\"color:red\">尿</span>中劳拉西泮的气相色谱-氮磷检测器的检测方法。检测时以2-羟乙基氟西泮为内标，用β-葡萄糖醛酸<span style=\"color:red\">苷</span>酶水解后于碱性条件下用乙醚萃取，将萃取液浓缩后进行检测。劳拉西泮的萃取率（mean±SD）为(83.4±3.1)% ，检出限为5 μg/L。萃取物用N，O-双(三甲硅烷基)三氟乙酰胺(BSTFA)衍生化后进行三甲基硅烷(TMS)衍生物检测。","authors":[{"authorName":"姜兆林","id":"45a9d8e3-2318-4da5-b2e8-f21d3ab2c410","originalAuthorName":"姜兆林"},{"authorName":"谭家镒","id":"27cc062f-a52b-470d-b264-57fdd16435dd","originalAuthorName":"谭家镒"},{"authorName":"姚丽娟","id":"a234d1a5-4b2a-449a-9779-535df1347ef8","originalAuthorName":"姚丽娟"},{"authorName":"邢丽梅","id":"4f280fb2-8e6b-4763-8181-8bb5f66e42eb","originalAuthorName":"邢丽梅"}],"doi":"10.3321/j.issn:1000-8713.2001.04.015","fpage":"341","id":"5a30e158-a3df-4699-b720-1e27f550910f","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"4a029185-1bde-4b4c-b366-689874ecfddf","keyword":"气相色谱法","originalKeyword":"气相色谱法"},{"id":"023459d3-169f-4c9e-825a-e5c68b9e63c2","keyword":"劳拉西泮","originalKeyword":"劳拉西泮"},{"id":"966973d3-7e76-4de3-a301-3850eaa54cbb","keyword":"<span style=\"color:red\">尿</span>","originalKeyword":"尿"}],"language":"zh","publisherId":"sp200104015","title":"气相色谱-氮磷检测法检测<span style=\"color:red\">尿</span>中劳拉西泮","volume":"19","year":"2001"},{"abstractinfo":"在不改变骨架的前提下提出一种从肌<span style=\"color:red\">苷</span>通过官能团转换制备腺苷的工艺方法,总收率可达62%.肌<span style=\"color:red\">苷</span>在醋酸钠存在下与醋酸酐发生酯化反应以92%的收率得到2′,3′,5′-三乙酰肌<span style=\"color:red\">苷</span>,其化合物经吡啶和三氯氧磷处理形成吡啶盐溶液,未分离直接在40 ℃、0.25～0.30 MPa压力下进行氨解反应以67%产率制得腺苷.腺苷结构经1H NMR、MS测试技术确证.这种简便的腺苷合成方法可适合于工业化生产.","authors":[{"authorName":"冀亚飞","id":"37e7cb54-6b29-46c0-b7ae-d7451bba5db3","originalAuthorName":"冀亚飞"},{"authorName":"许煦","id":"38f978b5-e7d0-49a2-a985-e6637b6927e6","originalAuthorName":"许煦"}],"doi":"10.3969/j.issn.1000-0518.2007.08.027","fpage":"971","id":"b9957121-d3f0-407f-ba41-96cfcb5c2d63","issue":"8","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"7a8a9c06-e179-4d18-8297-ce00f52b9fe2","keyword":"腺苷","originalKeyword":"腺苷"},{"id":"bcb9a808-073c-480a-9560-ccfb9e381a8f","keyword":"肌<span style=\"color:red\">苷</span>","originalKeyword":"肌苷"},{"id":"2c5c6d42-843d-4053-88de-554d49ce05da","keyword":"核苷","originalKeyword":"核苷"},{"id":"ead392ad-1fa2-4191-b79d-a629633abea6","keyword":"合成","originalKeyword":"合成"}],"language":"zh","publisherId":"yyhx200708027","title":"从肌<span style=\"color:red\">苷</span>简便合成腺苷","volume":"24","year":"2007"},{"abstractinfo":"建立了电泳-色谱扫描法检测<span style=\"color:red\">尿</span>中清蛋白含量的方法.该方法利用醋酸纤维薄膜电泳分离尿蛋白后,用作者合成的染色剂染色,用CS-930薄层扫描仪定量测定<span style=\"color:red\">尿</span>中清蛋白含量,其被测组分的质量浓度为0.3～3.6 g/L时与相应峰面积呈线性关系,相关系数为0.997 4.方法的精密度为4.20%,回收率为96.7%～104.3%.利用该方法对肾脏病人尿液中清蛋白的含量进行分析,快捷简便,灵敏度高,检出限低,线性范围宽,结果令人满意.","authors":[{"authorName":"曹晓峰","id":"3711196e-d8da-46cf-87e5-bd9debe15321","originalAuthorName":"曹晓峰"},{"authorName":"刁海鹏","id":"6acfaac3-416b-4a1b-bab0-1e8776dd0d29","originalAuthorName":"刁海鹏"},{"authorName":"卫建琮","id":"9362a773-c824-45be-9fea-33806dde09ac","originalAuthorName":"卫建琮"},{"authorName":"张生万","id":"921d2b0c-1b01-40d6-8753-9696b8d1f51a","originalAuthorName":"张生万"},{"authorName":"冯彦琳","id":"053ba75b-ec52-4893-9e7c-c6ab0e07f9d0","originalAuthorName":"冯彦琳"}],"doi":"10.3321/j.issn:1000-8713.2003.03.022","fpage":"270","id":"51e8594f-872c-4a5b-b132-53d4bd34625f","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"f3d9e64a-cf55-423f-acb7-146459f1af61","keyword":"色谱扫描","originalKeyword":"色谱扫描"},{"id":"9be0d35a-011a-4a25-a3e0-67534c4d63ed","keyword":"电泳","originalKeyword":"电泳"},{"id":"7bd86f35-dbdc-4995-86d5-9f037795b031","keyword":"清蛋白","originalKeyword":"清蛋白"},{"id":"fe1349eb-a513-4138-9060-7f6508b63df0","keyword":"<span style=\"color:red\">尿</span>","originalKeyword":"尿"}],"language":"zh","publisherId":"sp200303022","title":"电泳-色谱扫描法检测<span style=\"color:red\">尿</span>中清蛋白","volume":"21","year":"2003"},{"abstractinfo":"采用纳米粒度仪和TEM比较研究了<span style=\"color:red\">尿</span>石症患者和正常人尿液中<1000nm的纳米微晶的Zeta电位、光强自相关函数曲线、平均粒径和粒度分布.结果表明,在相近<span style=\"color:red\">尿</span>pH(6.0左右)时,<span style=\"color:red\">尿</span>石症患者尿液中纳米微晶的Zeta电位值(平均为-3.16mV)明显大于正常人(平均为-10.1mV).相比较正常人尿液,<span style=\"color:red\">尿</span>石症患者尿液中纳米微晶的散射光强存在更长时间的相关性,说明<span style=\"color:red\">尿</span>石症患者尿液中纳米微晶的粒径大于正常人.粒度分布检测进一步表明,正常人尿液中粒径在350nm以下的纳米微晶占大多数,而患者尿液中粒径>600nm的纳米微晶占大多数.实验结果有助于了解正常人尿液和<span style=\"color:red\">尿</span>石症患者尿液的差异,为<span style=\"color:red\">尿</span>石症的早期检测提供线索.","authors":[{"authorName":"黄志杰","id":"024743cc-679b-47d6-b083-948fb5b25bbb","originalAuthorName":"黄志杰"},{"authorName":"许小晶","id":"6a5e2e62-6063-47c8-a1ac-3d9a652a5f2d","originalAuthorName":"许小晶"},{"authorName":"邝荔","id":"31641bbb-7939-4066-b7c1-e6a2891a95ca","originalAuthorName":"邝荔"},{"authorName":"欧阳健明","id":"9a545a2d-6bb3-460b-9d52-05ff52fff032","originalAuthorName":"欧阳健明"}],"doi":"","fpage":"501","id":"b94aa077-1a46-44ec-a9b1-32c3847e926a","issue":"3","journal":{"abbrevTitle":"GNCL","coverImgSrc":"journal/img/cover/GNCL.jpg","id":"33","issnPpub":"1001-9731","publisherId":"GNCL","title":"功能材料"},"keywords":[{"id":"6bb8859d-c88c-466e-9769-e757c6b5fbf1","keyword":"纳米粒度仪","originalKeyword":"纳米粒度仪"},{"id":"0c87aa78-6cb5-4581-950b-363c5566d53e","keyword":"光强自相关函数","originalKeyword":"光强自相关函数"},{"id":"f5310188-e70f-4628-8b05-016cdc688e42","keyword":"TEM","originalKeyword":"TEM"},{"id":"7f54f36f-e498-4d0d-bfc5-f79f59f812aa","keyword":"粒度分布","originalKeyword":"粒度分布"},{"id":"b43929bc-ae40-47d9-9182-ed55d6508dfc","keyword":"Zeta电位","originalKeyword":"Zeta电位"}],"language":"zh","publisherId":"gncl200903044","title":"纳米粒度仪研究<span style=\"color:red\">尿</span>石症患者和正常人尿液中的纳米微晶","volume":"40","year":"2009"},{"abstractinfo":"建立了在线衍生、双波长高效液相色谱-荧光检测器同时检测血清中犬<span style=\"color:red\">尿</span>氨酸(kynurenine, Kyn)和犬<span style=\"color:red\">尿</span>喹啉酸(kynurenic acid, KYNA)含量的方法.血清标本经5%高氯酸溶液去除蛋白质后,上清液直接进样分析测定.采用的色谱柱为Hypersil C8柱;流动相为0.25 mol/L 醋酸锌-50 mmol/L 醋酸溶液(含3%乙腈),流速为1.5 mL/min.在0～10 min时间段,在激发波长和发射波长分别为365 nm和480 nm时检测Kyn;10 min后,在激发波长和发射波长分别变换为344 nm和404 nm时检测KYNA.Kyn的保留时间约为8.1 min,线性范围为98～19600 nmol/L,最低检出浓度为50 nmol/L,平均回收率为94.88% ,日内、日间测定值的相对标准偏差(RSD)均低于4% .KYNA的保留时间约为13.0 min,线性范围为2.62～1047 nmol/L,最低检出浓度为0.11 nmol/L,平均回收率为102.72% ,日内、日间测定的RSD均低于4% .苯丙氨酸、酪氨酸、色氨酸和 5-羟色胺等物质对目标物的检测无干扰.71例健康成人血清中,Kyn和KYNA含量分别为(1.40±0.34) μmol/L 和(24.22±8.67) nmol/L.该方法简便、快速、灵敏、特异,适于临床和科研应用.","authors":[{"authorName":"肖乐东","id":"d2c7105c-5cef-40fb-b12f-0d98cbcd727c","originalAuthorName":"肖乐东"},{"authorName":"唐爱国","id":"c5872c09-66f0-4bdf-a6ff-ecac2e41c606","originalAuthorName":"唐爱国"},{"authorName":"莫喜明","id":"6f496f86-5093-4c86-b445-1b5de331e610","originalAuthorName":"莫喜明"},{"authorName":"罗昔波","id":"a13525e6-67f3-40e4-b6fd-a975b6d196ea","originalAuthorName":"罗昔波"},{"authorName":"皮兰敢","id":"b52f6f0e-43c9-4714-8d37-b8af7610fc09","originalAuthorName":"皮兰敢"}],"doi":"10.3321/j.issn:1000-8713.2009.02.018","fpage":"220","id":"63051cdf-147e-4c82-8328-85afc74b84ad","issue":"2","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"05c8858f-3890-486d-b35b-17df65084c1b","keyword":"高效液相色谱法","originalKeyword":"高效液相色谱法"},{"id":"14c01161-269c-4a88-9f41-53ae6ea1ae50","keyword":"荧光检测","originalKeyword":"荧光检测"},{"id":"53781eb8-e7aa-4ec9-9b10-551f2615f644","keyword":"犬<span style=\"color:red\">尿</span>氨酸","originalKeyword":"犬尿氨酸"},{"id":"0a018052-0b0c-4b9b-a251-01a3a264375d","keyword":"犬<span style=\"color:red\">尿</span>喹啉酸","originalKeyword":"犬尿喹啉酸"},{"id":"489ce9b7-dca4-45fb-b784-7e525626aef4","keyword":"色氨酸","originalKeyword":"色氨酸"},{"id":"f98514fe-800d-45e0-9e55-85bb37535c73","keyword":"在线衍生","originalKeyword":"在线衍生"},{"id":"5d3319a7-d2a7-4368-b1d4-987b0fe788d7","keyword":"血清","originalKeyword":"血清"}],"language":"zh","publisherId":"sp200902018","title":"高效液相色谱-荧光检测法同时测定血清中的犬<span style=\"color:red\">尿</span>氨酸和犬<span style=\"color:red\">尿</span>喹啉酸","volume":"27","year":"2009"},{"abstractinfo":"研究了离子交换树脂催化薯蓣皂<span style=\"color:red\">苷</span>水解的过程. 通过正交试验、单因素试验研究了水解温度、水解时间、催化剂量等因素对薯蓣皂<span style=\"color:red\">苷</span>水解的影响,获得的优化反应条件为:反应温度80 ℃,反应时间9 h,催化剂量6 g对0.1 g皂<span style=\"color:red\">苷</span>. 实验表明,离子交换树脂催化皂<span style=\"color:red\">苷</span>水解过程的皂素得率约为盐酸催化水解的70%,溶液中的H+离子浓度仅为HCl催化水解的0.2%,可大大减轻现有催化剂导致的严重酸污染.","authors":[{"authorName":"李谦","id":"9c670b2c-d014-46c6-a16c-90c3e8ed1ec1","originalAuthorName":"李谦"},{"authorName":"高向涛","id":"f80691e6-578f-4ee3-98e3-932c246ffc10","originalAuthorName":"高向涛"},{"authorName":"徐俊清","id":"92f081fc-10ff-4c96-8d6a-7856b1c50cc9","originalAuthorName":"徐俊清"},{"authorName":"刘绣华","id":"ff642a86-2aaa-4840-94c6-9dc38c4763d3","originalAuthorName":"刘绣华"}],"doi":"10.3969/j.issn.1000-0518.2009.04.013","fpage":"431","id":"dd979a8c-9e13-4703-a7db-a35b8f6629c3","issue":"4","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"cbb695da-e093-4150-b7fa-1c4b653c3aa1","keyword":"薯蓣皂<span style=\"color:red\">苷</span>","originalKeyword":"薯蓣皂苷"},{"id":"b67e4056-4c56-4de5-b395-e020cb50b4fb","keyword":"薯蓣皂<span style=\"color:red\">苷</span>元","originalKeyword":"薯蓣皂苷元"},{"id":"846b7fc1-3929-46c7-bf90-87183c6b2ea1","keyword":"离子交换树脂","originalKeyword":"离子交换树脂"},{"id":"2c0911cb-d73d-4079-b45f-fc80b456bffe","keyword":"固体酸","originalKeyword":"固体酸"},{"id":"9f1fb272-37f6-4c49-b1ff-fc37e11b6a18","keyword":"正交设计","originalKeyword":"正交设计"}],"language":"zh","publisherId":"yyhx200904013","title":"离子交换树脂催化薯蓣皂<span style=\"color:red\">苷</span>水解","volume":"26","year":"2009"}],"totalpage":23,"totalrecord":229}