{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"重组人促红细胞生成素(rhEPO)是一种激素类<span style=\"color:red\">兴奋剂</span>,近年来被滥用在一些耐力性比赛项目中.由于重组与内源性EPO的氨基酸序列相同,区别很小,并且在尿样或血样中的浓度低,代谢快,给检测带来了很大的难度.本文从直接方法和间接方法两个方面综述了近几年来<span style=\"color:red\">兴奋剂</span>rhEPO及其类似物检测的研究进展,并结合本小组的工作展望了rhEPO检测的发展方向.","authors":[{"authorName":"杨霞","id":"1f2a3e99-c287-4d5e-8eab-b5e148e7ded3","originalAuthorName":"杨霞"},{"authorName":"庞楠楠","id":"27980815-19e3-4906-a360-dbaacdb88f9c","originalAuthorName":"庞楠楠"},{"authorName":"廖一平","id":"524c20c0-fbd1-4eef-aa3d-e02ec5cfe6ea","originalAuthorName":"廖一平"},{"authorName":"刘虎威","id":"f0ff1f37-da51-42f0-ac50-446be81e6d88","originalAuthorName":"刘虎威"}],"doi":"","fpage":"413","id":"ecf2047c-8840-4440-8c37-ed97e9fefcb9","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"2203af06-1cd4-42ab-8e18-854f21b2ba65","keyword":"重组人促红细胞生成素","originalKeyword":"重组人促红细胞生成素"},{"id":"c410c36e-a6a3-4e13-ba4a-e5ab3a993dfa","keyword":"<span style=\"color:red\">兴奋剂</span>","originalKeyword":"兴奋剂"},{"id":"f37eb8d9-de26-4928-9ff2-87aadaedf1b6","keyword":"检测","originalKeyword":"检测"},{"id":"81ceaee9-6a96-49ee-9bc6-0eaeb8a596a5","keyword":"直接方法","originalKeyword":"直接方法"},{"id":"06839d03-4987-4801-93ae-5aef35c05b95","keyword":"间接方法","originalKeyword":"间接方法"},{"id":"2e701a00-6e28-4ac1-8e93-8917c7135ad8","keyword":"综述","originalKeyword":"综述"}],"language":"zh","publisherId":"sp200804004","title":"<span style=\"color:red\">兴奋剂</span>重组人促红细胞生成素及其类似物检测方法的研究进展","volume":"26","year":"2008"},{"abstractinfo":"建立了一种简单、灵敏、快速地同时测定人体尿液中3类(刺激<span style=\"color:red\">剂</span>、麻醉<span style=\"color:red\">剂</span>和抗雌激素)5种<span style=\"color:red\">兴奋剂</span>的气相色谱-氮磷检测(GC-NPD)方法.尿样采用非衍生法液-液萃取预处理技术,选用叔丁基甲醚作为萃取溶剂,二苯胺作为内标进行定量检测.即在一定量标准品及内标的混合溶液中加入5.0 mL空白尿样混合均匀后,加入0.5 mL 5 mol/L 氢氧化钠溶液、3.0 g 氯化钠和5.0 mL叔丁基甲醚提取液,涡旋、离心,萃取液经氮气吹干、丙酮溶解后用GC-NPD测定.该方法在0.022～20 mg/L之间呈现良好的线性关系,相关系数为 0.9945～0.9998 ,最小检出质量浓度为0.007～0.015 mg/L.5种<span style=\"color:red\">兴奋剂</span>的尿样加标回收率为75.8% ～118.2%,相对标准偏差小于17.2% .","authors":[{"authorName":"邱丽君","id":"9f9c0337-77c4-4110-9136-46d45f11d3d0","originalAuthorName":"邱丽君"},{"authorName":"郑小严","id":"672bfebc-e4c0-4419-b310-b66fdefe5be0","originalAuthorName":"郑小严"},{"authorName":"游飞明","id":"d6ef3d15-c6da-4989-92e4-2a1929180ad3","originalAuthorName":"游飞明"},{"authorName":"刘薇","id":"859346a8-fe12-4989-9a5c-ffcd1c37df62","originalAuthorName":"刘薇"},{"authorName":"张金章","id":"21b4b4e8-df8b-4505-8d00-2e2b1ead4cc9","originalAuthorName":"张金章"},{"authorName":"张兰","id":"0df94457-8c38-4c3c-b3da-33ad9ebb57ba","originalAuthorName":"张兰"}],"doi":"10.3321/j.issn:1000-8713.2009.03.023","fpage":"364","id":"f19377ea-b8da-4800-b79a-ac92c13ca651","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"43b087a0-dca1-4023-8194-1ee07bbd5953","keyword":"气相色谱-氮磷检测","originalKeyword":"气相色谱-氮磷检测"},{"id":"0e1944c6-e5fa-4064-9b33-14cf5c61f028","keyword":"刺激<span style=\"color:red\">剂</span>","originalKeyword":"刺激剂"},{"id":"29cbee87-8893-45ff-b810-6982890c9bcb","keyword":"麻醉<span style=\"color:red\">剂</span>","originalKeyword":"麻醉剂"},{"id":"9065c789-283b-45e1-8586-11d1b932a160","keyword":"抗雌激素","originalKeyword":"抗雌激素"},{"id":"9fd67cc9-4059-4b5d-aebd-7d5c1ecb41a1","keyword":"<span style=\"color:red\">兴奋剂</span>","originalKeyword":"兴奋剂"},{"id":"afd3075e-a6b1-4ad7-9fdb-07570caa0f24","keyword":"尿","originalKeyword":"尿"}],"language":"zh","publisherId":"sp200903023","title":"气相色谱-氮磷检测方法同时检测尿样中的刺激<span style=\"color:red\">剂</span>、麻醉<span style=\"color:red\">剂</span>和抗雌激素类五种<span style=\"color:red\">兴奋剂</span>","volume":"27","year":"2009"},{"abstractinfo":"采用溶胶-凝胶法制备了-种双缩水甘油基杯[4]芳烃羟基硅油探头,运用自行设计的反萃取装置实现了顶空固相微萃取与毛细管电泳的离线联用,结合超声反萃取和场放大进样技术成功地测定了尿样中<span style=\"color:red\">兴奋剂</span>普萘洛尔异构体的含量.实验考察了固相微萃取条件和反萃取条件对测定结果的影响,比较了杯[4]芳烃探头与商品化探头对于尿样中普萘洛尔异构体的萃取性能,结果表明基于杯[4]芳烃探头的固相微萃取过程能够实现满意的净化效应与预富集效应.利用毛细管电泳-二极管阵列检测器对加标尿样中普萘洛尔异构体测定的线性范围为0.05～10 mg/L,检测限为8～10μg/L,相对标准偏差小于6.5%(n=6),两种异构体的加标回收率为86%～107%(n=5).该探头的可重复使用(＞150次)性能良好.","authors":[{"authorName":"周兴旺","id":"ef7b4ecd-9b03-4a93-aedb-82b050e61448","originalAuthorName":"周兴旺"},{"authorName":"李秀娟","id":"b3321339-9dc6-4787-80b5-accff7179a88","originalAuthorName":"李秀娟"},{"authorName":"曾昭睿","id":"7cf7bfee-bd5e-43bb-bc5e-9a035e042ecc","originalAuthorName":"曾昭睿"}],"doi":"10.3321/j.issn:1000-8713.2006.01.001","fpage":"1","id":"47ac8b8f-d646-413a-b013-372408715caa","issue":"1","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"7cee55e0-2ec5-4f34-a7be-6b4219aa3600","keyword":"杯[4]芳烃","originalKeyword":"杯[4]芳烃"},{"id":"834085c8-9e0c-4bf2-97b8-492455e14ccb","keyword":"固相微萃取","originalKeyword":"固相微萃取"},{"id":"cb9ffc4d-08b9-41be-a6e2-4263a86705c7","keyword":"毛细管电泳","originalKeyword":"毛细管电泳"},{"id":"35f6a40a-5e3f-45bb-b1ca-4eba711f6575","keyword":"<span style=\"color:red\">兴奋剂</span>","originalKeyword":"兴奋剂"},{"id":"377a5f88-c7bc-41fc-947b-7c593ac9fa73","keyword":"普萘洛尔异构体","originalKeyword":"普萘洛尔异构体"}],"language":"zh","publisherId":"sp200601001","title":"基于杯[4]芳烃探头的固相微萃取-毛细管电泳法测定尿样中的<span style=\"color:red\">兴奋剂</span>普萘洛尔异构体","volume":"24","year":"2006"},{"abstractinfo":"生物技术在取得巨大成就的同时也导致了<span style=\"color:red\">兴奋剂</span>的发展.从体外重组蛋白到潜在的基因<span style=\"color:red\">兴奋剂</span>的出现,给<span style=\"color:red\">兴奋剂</span>检测工作带来了巨大的挑战.基因治疗的发展以及一些功能基因对运动技能提高的潜力,加大了基因<span style=\"color:red\">兴奋剂</span>使用的可能性.本文在介绍基因<span style=\"color:red\">兴奋剂</span>的基础上,阐述了基因<span style=\"color:red\">兴奋剂</span>检测方法的研究现状,展望了基因<span style=\"color:red\">兴奋剂</span>检测技术的发展策略.","authors":[{"authorName":"王文珺","id":"d64069e7-b01d-4268-8aa7-bfc14daba5e0","originalAuthorName":"王文珺"},{"authorName":"张四纯","id":"1617f49e-9627-4490-a147-76875b9f1494","originalAuthorName":"张四纯"},{"authorName":"徐静娟","id":"6f0db9aa-5a5e-4ced-aad1-911e1c7a18a8","originalAuthorName":"徐静娟"},{"authorName":"夏兴华","id":"4d06e8ef-b2ae-47c7-8486-9ed1dc5c378d","originalAuthorName":"夏兴华"},{"authorName":"田亚平","id":"e65b88e6-321d-4b6c-9729-b0e5b7d605e6","originalAuthorName":"田亚平"},{"authorName":"张新荣","id":"f542a8e7-8145-4eec-a12c-db6420ee69be","originalAuthorName":"张新荣"},{"authorName":"陈洪渊","id":"da4598ce-7888-4332-8eff-06e609d95990","originalAuthorName":"陈洪渊"}],"doi":"","fpage":"408","id":"56e7eaaa-c7a2-4f8c-9b31-b5a590793eca","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"947b31c2-8481-47e5-99ad-9053710064f5","keyword":"<span style=\"color:red\">兴奋剂</span>检测","originalKeyword":"兴奋剂检测"},{"id":"94e5c5a8-0eea-48af-acb0-3f14dc88d6dd","keyword":"基因<span style=\"color:red\">兴奋剂</span>","originalKeyword":"基因兴奋剂"},{"id":"1568d271-9260-4843-b345-8d44abe3625b","keyword":"基因治疗","originalKeyword":"基因治疗"},{"id":"ed9fc2aa-dc85-46eb-9836-0f6db61efae6","keyword":"促红细胞生长素","originalKeyword":"促红细胞生长素"},{"id":"5673a547-70ba-4652-a70d-2de62bb9947e","keyword":"胰岛素样生长因子-I","originalKeyword":"胰岛素样生长因子-I"}],"language":"zh","publisherId":"sp200804003","title":"基因<span style=\"color:red\">兴奋剂</span>检测的现状与展望","volume":"26","year":"2008"},{"abstractinfo":"本文综述了基因<span style=\"color:red\">兴奋剂</span>检测的现状和反基因<span style=\"color:red\">兴奋剂</span>的策略.归纳了可能被运动员滥用的基因<span style=\"color:red\">兴奋剂</span>,分析了由这些基因表达的促红细胞生成素(EPO)、人生长激素(hGH)等蛋白的检测进展,讨论了未来检测基因<span style=\"color:red\">兴奋剂</span>的可能策略.","authors":[{"authorName":"何坚刚","id":"6acc4eeb-e068-4d51-84b8-33d43bab95fb","originalAuthorName":"何坚刚"},{"authorName":"刘震","id":"8900e3e2-2509-416a-bf6d-27a28f7f4e08","originalAuthorName":"刘震"},{"authorName":"刘晶","id":"64c14123-0207-4a74-93f6-636eee1d7819","originalAuthorName":"刘晶"},{"authorName":"窦鹏","id":"00a90a18-487a-4390-ad2b-789771b4d412","originalAuthorName":"窦鹏"},{"authorName":"陈洪渊","id":"87914f84-db34-45b1-95a7-93347f7fbea6","originalAuthorName":"陈洪渊"}],"doi":"","fpage":"402","id":"e5d2a46a-809d-4f7a-a10a-9483a068e3c5","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"60cff400-3f11-4954-bb20-06964ed3be0d","keyword":"基因<span style=\"color:red\">兴奋剂</span>","originalKeyword":"基因兴奋剂"},{"id":"36d01b0e-cfd0-4706-8fb2-5dde3ad15d3c","keyword":"重组蛋白","originalKeyword":"重组蛋白"},{"id":"c5828de5-36fb-4d07-b023-0df818da86ae","keyword":"检测","originalKeyword":"检测"},{"id":"94d69130-5cad-4d0b-9b0e-f831c323d552","keyword":"综述","originalKeyword":"综述"}],"language":"zh","publisherId":"sp200804002","title":"基因<span style=\"color:red\">兴奋剂</span>检测的现状与策略","volume":"26","year":"2008"},{"abstractinfo":"液相色谱-质谱联用技术已越来越广泛地应用在<span style=\"color:red\">兴奋剂</span>的检测中,其中包括对各类小分子<span style=\"color:red\">兴奋剂</span>和肽类激素等的检测.本文综述了近年来液相色谱-质谱联用在<span style=\"color:red\">兴奋剂</span>检测中的筛选、确证和定量方面的应用及进展情况,并讨论了相关的检测标准.","authors":[{"authorName":"秦旸","id":"6ce3f4e6-3bf3-48ba-bc10-bda0bc1f80a0","originalAuthorName":"秦旸"},{"authorName":"徐友宣","id":"6388f699-fae7-4010-8736-47f2db39da7f","originalAuthorName":"徐友宣"},{"authorName":"杨树民","id":"a6c45cbc-16bb-4cb2-a4f0-363f96848c53","originalAuthorName":"杨树民"},{"authorName":"朱绍棠","id":"e699a39b-8370-4c8c-a3fd-f4375aa92fd7","originalAuthorName":"朱绍棠"}],"doi":"","fpage":"431","id":"2316fd0d-ab47-43a8-87e8-7ac92a93bc76","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"1d92d1d0-e4c4-4e5c-96bd-d0b0a04aab96","keyword":"液相色谱-质谱","originalKeyword":"液相色谱-质谱"},{"id":"595b5291-5ec0-4d85-9f84-1228fffa8f10","keyword":"<span style=\"color:red\">兴奋剂</span>检测","originalKeyword":"兴奋剂检测"},{"id":"087c95b6-014e-4ab1-ae1f-acfa0e4dc37b","keyword":"综述","originalKeyword":"综述"}],"language":"zh","publisherId":"sp200804007","title":"液相色谱-质谱联用在<span style=\"color:red\">兴奋剂</span>检测中的应用及进展","volume":"26","year":"2008"},{"abstractinfo":"蛋白同化雄性类固醇是一类滥用最为普遍的<span style=\"color:red\">兴奋剂</span>物质,对其进行有效的控制和检测关系到运动员的身心健康和体育比赛的公平公正.对类固醇<span style=\"color:red\">兴奋剂</span>分析方法的改进和发展是目前<span style=\"color:red\">兴奋剂</span>检测的重要任务.本文主要是对自2002年以来类固醇<span style=\"color:red\">兴奋剂</span>样品的预处理和检测手段的研究进展做一概述,包括气相色谱-质谱法、液相色谱-质谱法、免疫法、电化学方法以及质谱法等.","authors":[{"authorName":"龙媛媛","id":"f11b95c0-e3b4-477b-9dd1-e27cd2a01d45","originalAuthorName":"龙媛媛"},{"authorName":"王丁众","id":"108e7f72-7fec-4602-825b-29bf507a45b3","originalAuthorName":"王丁众"},{"authorName":"李克安","id":"59acd734-1034-498f-a4d3-a620bab43a49","originalAuthorName":"李克安"},{"authorName":"刘锋","id":"4266e6a7-2363-4792-bfa1-a484170f55c9","originalAuthorName":"刘锋"}],"doi":"","fpage":"417","id":"c747c418-92d5-402d-bad8-6b676651f769","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"0db0a974-6aa1-4e7d-b6ac-3106e4a79467","keyword":"类固醇","originalKeyword":"类固醇"},{"id":"ad000aea-b34f-40a1-a74f-e2f3808b6eda","keyword":"反<span style=\"color:red\">兴奋剂</span>","originalKeyword":"反兴奋剂"},{"id":"0f51fe93-9ed4-401d-b823-0f32abfbd57a","keyword":"分析检测","originalKeyword":"分析检测"},{"id":"457cdb24-6b8a-4e11-ab76-118621be5efe","keyword":"样品预处理","originalKeyword":"样品预处理"}],"language":"zh","publisherId":"sp200804005","title":"类固醇<span style=\"color:red\">兴奋剂</span>分析方法的研究进展","volume":"26","year":"2008"},{"abstractinfo":"建立了液相色谱-串联质谱(LC-MS/MS)测定尿液中的内源性类固醇激素的方法.尿样经葡萄糖醛酸甙酶酶解后进行液-液提取,以甲醇-0.1%甲酸缓冲液(含0.02 mol/L乙酸铵)(体积比为68∶32)为流动相,采用Cosmosil C18色谱柱分离,并以三重四极杆串联质谱多反应监测扫描方式对尿样中的脱氢表雄酮(DHEA)、睾酮、表睾酮、雄酮和苯胆烷醇酮等5种激素进行检测.方法的最低检出限为0.01～10 ng/mL,平均回收率为96.7%～106.5%,日内和日间相对标准偏差(RSD)分别小于7%和11%.应用所建立的方法测定了健康志愿者口服DHEA后尿液中内源性类固醇激素的变化情况,结果表明该方法样品处理简便,色谱分离完全,结果准确可靠,可替代气相色谱-质谱法用于体液中内源性类固醇激素<span style=\"color:red\">兴奋剂</span>的常规分析.","authors":[{"authorName":"王萌烨","id":"468b7748-69c3-4352-8ebf-a834bf09f537","originalAuthorName":"王萌烨"},{"authorName":"向平","id":"b49b072e-d080-40df-b571-96d086b35465","originalAuthorName":"向平"},{"authorName":"严慧","id":"631f88e7-9994-4107-80a7-55d1f44b77f2","originalAuthorName":"严慧"},{"authorName":"沈保华","id":"2601f0dd-4106-469d-83fa-8774de754909","originalAuthorName":"沈保华"},{"authorName":"沈敏","id":"58218c4d-445a-4430-9865-a737959adc9e","originalAuthorName":"沈敏"}],"doi":"10.3321/j.issn:1000-8713.2008.01.003","fpage":"10","id":"9bf9edde-a995-4b6e-9910-aa6878623b4e","issue":"1","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"ac3b997f-52ae-48e6-9415-fad63e80b9af","keyword":"液相色谱-串联质谱法","originalKeyword":"液相色谱-串联质谱法"},{"id":"1814b6e2-acd9-4472-a224-b690a7fe8536","keyword":"内源性类固醇激素","originalKeyword":"内源性类固醇激素"},{"id":"1469ec56-680f-4533-9c74-e1b0a00b6cfc","keyword":"脱氢表雄酮","originalKeyword":"脱氢表雄酮"},{"id":"2440e8a2-a899-4d23-8f12-ea30ec895f9d","keyword":"<span style=\"color:red\">兴奋剂</span>","originalKeyword":"兴奋剂"},{"id":"bfa63897-86f7-41fb-b3ea-52df0518fba2","keyword":"尿液","originalKeyword":"尿液"}],"language":"zh","publisherId":"sp200801003","title":"液相色谱-串联质谱法测定尿液中的内源性类固醇激素","volume":"26","year":"2008"},{"abstractinfo":"建立了一种同时分离检测包括利尿<span style=\"color:red\">剂</span>、蛋白同化<span style=\"color:red\">剂</span>、β-阻断<span style=\"color:red\">剂</span>、麻醉<span style=\"color:red\">剂</span>、β2-激动<span style=\"color:red\">剂</span>、刺激<span style=\"color:red\">剂</span>等6类8种<span style=\"color:red\">兴奋剂</span>的毛细管区带电泳-紫外检测法.优化的色谱条件为:以50 mmol/L 甲酸铵-氨水(pH 7.8)缓冲液为运行液,于3 kPa下进样10 s,分离电压为20 kV,检测波长为214 nm.在此条件下,8种<span style=\"color:red\">兴奋剂</span>在 7 min 内实现了快速的基线分离.在相应的浓度范围内,8种组分的浓度与峰高呈良好的线性关系,检出限达为0.2~0.7 μg/mL.该方法快速,分析成本低,无污染,非常适用于多种类<span style=\"color:red\">兴奋剂</span>的同时快速检测.","authors":[{"authorName":"肖惠","id":"76c0dda5-1472-4445-8942-b2255998224d","originalAuthorName":"肖惠"},{"authorName":"童萍","id":"edebfd89-e1f6-4b9c-b74f-881d2d54864e","originalAuthorName":"童萍"},{"authorName":"冯强","id":"88cbb9ad-969d-466e-85c3-aae44e3e79c8","originalAuthorName":"冯强"},{"authorName":"张兰","id":"5f621afd-1ffa-4585-b4c8-64c8abf21ccb","originalAuthorName":"张兰"}],"doi":"","fpage":"444","id":"509a16d7-0589-4aa0-85e1-88154edbb376","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"92ed0f91-8a6f-4c29-afb1-4e56e8d0a49f","keyword":"毛细管区带电泳","originalKeyword":"毛细管区带电泳"},{"id":"18d71729-3815-4c08-a75a-510cfad31832","keyword":"多种类<span style=\"color:red\">兴奋剂</span>","originalKeyword":"多种类兴奋剂"},{"id":"274dcc3f-295e-4607-af5e-e55c7f15141f","keyword":"同时检测","originalKeyword":"同时检测"}],"language":"zh","publisherId":"sp200804009","title":"毛细管区带电泳用于多种类<span style=\"color:red\">兴奋剂</span>的同时快速分离检测","volume":"26","year":"2008"},{"abstractinfo":"建立了饲料中克仑特罗、莱克多巴胺、喷布特罗、妥布特罗等18种β-<span style=\"color:red\">兴奋剂</span>的 QuEChERS 结合高效液相色谱-串联质谱的检测方法。饲料样品加水分散后经4％（ v / v）氨水乙腈提取，加入25 mg 十八烷基硅烷（ C 18）和50 mg N-丙基乙二胺（PSA）吸附<span style=\"color:red\">剂</span>分散固相萃取净化后，以高效液相色谱-串联质谱进行定性和定量分析。采用 Agi-lent Zorbax Eclipse XDB-C 18（50 mm×4.6 mm，1.8μm）分析柱，以甲醇-0.1％（v / v）甲酸水溶液为流动相进行梯度洗脱，串联质谱在多反应监测（MRM）正离子模式下进行检测，基质外标法定量。结果表明，18种待测物在质量浓度为5~200μg / L 范围内线性关系良好，相关系数为0.9912~0.9995；在0.05、0.1、0.5 mg / kg 3个浓度加标水平下，饲料中18种β-<span style=\"color:red\">兴奋剂</span>的平均回收率为78.4％~107.1％，相对标准偏差（RSD）为3.5％~12.3％，定量限（以信噪比≥10计）均为0.05 mg / kg。该方法准确、灵敏，前处理简单，可作为饲料中克仑特罗等18种β-<span style=\"color:red\">兴奋剂</span>筛选和确认的检测方法。","authors":[{"authorName":"郑玲","id":"afd95ef8-b762-4ea9-a7e0-a348e8b36fa6","originalAuthorName":"郑玲"},{"authorName":"吴玉杰","id":"1b4ceed2-066c-4e11-98a5-a0ab0f1848f2","originalAuthorName":"吴玉杰"},{"authorName":"赵永锋","id":"3962f88e-d781-441b-85c9-d24a0a38a87c","originalAuthorName":"赵永锋"},{"authorName":"李丽华","id":"e6555058-1bd8-4c40-84a4-051392c5a372","originalAuthorName":"李丽华"},{"authorName":"马燕娟","id":"d1d6dd41-4e5d-417b-adb3-ca0cec8e7c81","originalAuthorName":"马燕娟"}],"doi":"10.3724/SP.J.1123.2014.03029","fpage":"867","id":"5c73e8d3-9539-4695-97f2-a6d190468a5a","issue":"8","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"08f79fd9-a3e8-417c-8311-490a5927eaec","keyword":"QuEChERS","originalKeyword":"QuEChERS"},{"id":"6210b04f-8a72-40f1-aa7b-72e2c67f0544","keyword":"高效液相色谱-串联质谱","originalKeyword":"高效液相色谱-串联质谱"},{"id":"221bc5c9-ecf0-42fe-9a2b-95d8c4bcf312","keyword":"β-<span style=\"color:red\">兴奋剂</span>","originalKeyword":"β-兴奋剂"},{"id":"64571d55-c05a-46af-9769-b2fbfe6941f3","keyword":"饲料","originalKeyword":"饲料"}],"language":"zh","publisherId":"sp201408013","title":"QuEChERS 结合高效液相色谱-串联质谱法测定饲料中的18种β-<span style=\"color:red\">兴奋剂</span>","volume":"","year":"2014"}],"totalpage":3018,"totalrecord":30171}